Literature summary extracted from

Kopriva, S.; Buchert, T.; Fritz, G.; Suter, M.; Weber, M.; Benda, R.; Schaller, J.; Feller, U.; Schurmann, P.; Schunemann, V.; Trautwein, A.X.; Kroneck, P.M.; Brunold, C.
Plant adenosine 5'-phosphosulfate reductase is a novel iron-sulfur protein (2001), J. Biol. Chem., 276, 42881-42886.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.8.99.2	overexpression in Escherichia coli	Arabidopsis thaliana
1.8.99.2	overexpression in Escherichia coli	Lemna minor

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.8.99.2	C129S	maximal velocity dropps to 50% of that of the wild-type enzyme	Lemna minor
1.8.99.2	C248S	mutation completely abolishes enzyme activity	Lemna minor

General Stability

EC Number	General Stability	Organism
1.8.99.2	enzyme is rapidly denatured by even micromolar concentrations of ferricyanide	Lemna minor

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.8.99.2	Iron	4 paramagnetic iron sites, which form a diamagnetic [4Fe-4S]2+ cluster	Lemna minor

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.99.2	Arabidopsis thaliana	-	-	-
1.8.99.2	Lemna minor	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.8.99.2	30	40	-	Lemna minor

Storage Stability

EC Number	Storage Stability	Organism
1.8.99.2	4°C, continous loss of activity	Lemna minor
1.8.99.2	4°C, continous loss of activity, precipitation after 2-3 days	Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.8.99.2	adenylyl sulfate + dithioerythritol	-	Lemna minor	AMP + sulfite + ?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.8.99.2	ATAPR2	-	Arabidopsis thaliana
1.8.99.2	LMAPR	-	Lemna minor

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.8.99.2	flavin	flavin is absent	Arabidopsis thaliana
1.8.99.2	flavin	flavin is absent	Lemna minor

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Release 2023.1 (January 2023)