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Literature summary extracted from

  • Fritz, G.; Roth, A.; Schiffer, A.; Buchert, T.; Bourenkov, G.; Bartunik, H.D.; Huber, H.; Stetter, K.O.; Kroneck, P.M.H.; Ermler, U.
    Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6-A resolution (2002), Proc. Natl. Acad. Sci. USA, 99, 1836-1841.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.99.2
-
 Archaeoglobus fulgidus
1.8.99.2 the structures of the enzyme in the two-electron reduced state and with sulfite bound to FAD are reported at 1.6 A and 2.5 A resolution, respectively Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.99.2 4Fe-4S cluster flavoenzyme.The two electrons required for adenylyl sulfate reduction are transferred via two [4Fe-4S] clusters from the surface of the protein to FAD. The large difference in reduction potential of these clusters (-60 mV and -500 mV) can be explained by interactions of the clusters with the protein matrix Archaeoglobus fulgidus
1.8.99.2 Iron the two electrons required for the adenylyl sulfate reduction are transferred via two [4Fe-4S] clusters from the surface of the protein to FAD Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.2 Archaeoglobus fulgidus
-
-
-
1.8.99.2 Archaeoglobus fulgidus O28603
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.99.2 AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor catalysis involves a nucleophilic attack of the N5-atom of reduced FAD on the sulfur atom of the enzyme Archaeoglobus fulgidus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 adenylyl sulfate + reduced acceptor
-
 Archaeoglobus fulgidus AMP + sulfite + acceptor
-
 r
1.8.99.2 AMP + sulfite + acceptor
-
 Archaeoglobus fulgidus adenylyl sulfate + reduced acceptor
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.8.99.2 adenosine 5'-phosphosulfate reductase
-
 Archaeoglobus fulgidus
1.8.99.2 APS reductase
-
 Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.2 FAD flavoenzyme.The two electrons required for adenylyl sulfate reduction are transferred via two [4Fe-4S] clusters from the surface of the protein to FAD Archaeoglobus fulgidus
1.8.99.2 flavin flavoenzyme Archaeoglobus fulgidus