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Literature summary extracted from

  • Mitchell, D.J.; Nikolic, D.; Jang, M.H.; van Breemen, R.B.; Hille, R.; Silverman, R.B.
    Inactivation of C30A trimethylamine dehydrogenase by N-cyclopropyl-alpha-methylbenzylamine, 1-phenylcyclopropylamine, and phenylhydrazine (2001), Biochemistry, 40, 8523-8530.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.2 expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.8.2 C30A binds FMN non-covalently Methylophilus methylotrophus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.2 1-phenylcyclopropylamine irreversible inactivation of both wild-type and mutant C30A Methylophilus methylotrophus
1.5.8.2 N-cyclopropyl-alpha-methylbenzylamine irreversible inactivation of both wild-type and mutant C30A, more potent than 1-phenylcyclopropylamine Methylophilus methylotrophus
1.5.8.2 phenylhydrazine irreversible inactivation of both wild-type and mutant C30A, 10 times more potent than N-cyclopropyl-alpha-methylbenzylamine Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.2 Fe
-
Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus
-
dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus W3A1
-
dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, recombinant enzyme Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
Methylophilus methylotrophus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
Methylophilus methylotrophus dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.2 FMN
-
Methylophilus methylotrophus