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Literature summary extracted from

  • Basran, J.; Chohan, K.K.; Sutcliffe, M.J.; Scrutton, N.S.
    Differential coupling through Val-344 and Tyr-442 of trimethylamine dehydrogenase in electron transfer reactions with ferricenium ions and electron transferring flavoprotein (2000), Biochemistry, 39, 9188-9200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.2 expression in Escherichia coli Methylophilus methylotrophus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.8.2
-
 Methylophilus methylotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.8.2 V344I increase of Km-value, decrease of turnover Methylophilus methylotrophus
1.5.8.2 Y442G
-
 Methylophilus methylotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.2 0.0148
-
 trimethylamine wild-type form, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 0.0242
-
 trimethylamine mutant V344I, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 0.0659
-
 trimethylamine mutant Y442G, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.2 Fe 4Fe-4S-center Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus W3A1
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, recombinant enzyme Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
 Methylophilus methylotrophus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.8.2 0.5
-
 trimethylamine mutant Y442G, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 6.7
-
 trimethylamine mutant V344I, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 16.8
-
 trimethylamine electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.2 FMN
-
 Methylophilus methylotrophus