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Literature summary extracted from

  • Ertrughrul, O.W.D.; Errington, N.; Raza, S.; Sutcliffe, M.J.; Rowe, A.J.; Scrutton, N.S.
    Probing the stabilizing role of C-terminal residues in trimethylamine dehydrogenase (1998), Protein Eng., 11, 447-455.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.2 expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.8.2 C-terminal truncations C-terminal truncations of 5, 10 or 17 residues do not affect dimer stability, but compromise the ability of the enzyme to become covalently coupled with FMN in the active site Methylophilus methylotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.2 0.0046
-
 trimethylamine mutant DELTA725-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 0.0047
-
 trimethylamine mutant DELTA720-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 0.0096
-
 trimethylamine recombinant wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 0.0137
-
 trimethylamine native wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.2 Fe 4Fe-4S-center Methylophilus methylotrophus
1.5.8.2 Fe 4.5 mol iron per mol enzyme Methylophilus methylotrophus

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, chromatography steps Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
 Methylophilus methylotrophus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.8.2 trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.8.2 trimethylamine + H2O + phenazine methosulfate
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 trimethylamine + H2O + phenazine methosulfate
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.8.2 5.6
-
 trimethylamine mutant delta725-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 6.4
-
 trimethylamine mutant delta720-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 10.2
-
 trimethylamine recombinant wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 15.6
-
 trimethylamine native wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.2 ADP
-
 Methylophilus methylotrophus
1.5.8.2 FMN
-
 Methylophilus methylotrophus