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Literature summary extracted from

  • Wilson, E.K.; Huang, L.; Sutcliffe, M.J.; Mathews, F.S.; Hille, R.; Scrutton, N.S.
    An exposed tyrosine on the surface of trimethylamine dehydrogenase facilitates electron transfer of electron transferring flavoprotein: Kinetics of transfer in wild-type and mutant complexes (1997), Biochemistry, 36, 41-48.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.2 expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.8.2 Y442F 1.4fold reduction of electron transfer Methylophilus methylotrophus
1.5.8.2 Y442G 30.5fold reduction of electron transfer Methylophilus methylotrophus
1.5.8.2 Y442L 2.2fold reduction of electron transfer Methylophilus methylotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.2 0.0143
-
 trimethylamine mutant Y442F, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 0.0175
-
 trimethylamine wild-type form, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 0.0196
-
 trimethylamine mutant Y442L, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 0.0262
-
 trimethylamine mutant Y442G, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.2 Fe 4Fe-4S-center Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus W3A1
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, chromatography steps Methylophilus methylotrophus
1.5.8.2 to homogeneity, recombinant enzyme Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
 Methylophilus methylotrophus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.8.2 0.25
-
 trimethylamine mutant Y442G, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 3.8
-
 trimethylamine mutant Y442L, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 10.3
-
 trimethylamine mutant Y442F, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus
1.5.8.2 16.5
-
 trimethylamine wild-type form, electron acceptor: electron transferring flavoprotein Methylophilus methylotrophus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.2 FMN
-
 Methylophilus methylotrophus