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Literature summary extracted from

  • Scrutton, N.S.; Packman, L.C.; Mathews, F.S.; Rohlfs, R.J.; Hille, R.
    Assembly of redox centers in the trimethylamine dehydrogenase of bacterium W3A1. Properties of the wild-type enzyme and a C30A mutant expressed from a cloned gene in Escherichia coli (1994), J. Biol. Chem., 269, 13942-13950.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.2 expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.8.2 C30A binds FMN non-covalently Methylophilus methylotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.2 0.017
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate, wild-type enzyme Methylophilus methylotrophus
1.5.8.2 0.019
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate, recombinant enzyme Methylophilus methylotrophus
1.5.8.2 2
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate, mutant C30A Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.2 Fe 4Fe-4S-center Methylophilus methylotrophus

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, chromatography steps Methylophilus methylotrophus
1.5.8.2 to homogeneity, recombinant enzyme Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
 Methylophilus methylotrophus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 trimethylamine + H2O + ferricenium hexafluorophosphate
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + ferrocenium hexafluorophosphate
-
 ?
1.5.8.2 trimethylamine + H2O + ferricenium hexafluorophosphate
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + ferrocenium hexafluorophosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.8.2 17
-
 trimethylamine mutant C30A, electron acceptor: ferricenium hexafluorophosphate Methylophilus methylotrophus
1.5.8.2 38
-
 trimethylamine electron acceptor: ferricenium hexafluorophosphate Methylophilus methylotrophus
1.5.8.2 40
-
 trimethylamine recombinant enzyme, electron acceptor: ferricenium hexafluorophosphate Methylophilus methylotrophus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.2 ADP
-
 Methylophilus methylotrophus
1.5.8.2 FMN covalently bound to Cys30 Methylophilus methylotrophus