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Literature summary extracted from

  • Huang, L.; Scrutton, N.S.; Hille, R.
    Reaction of the C30A mutant of trimethylamine dehydrogenase with diethylmethylamine (1996), J. Biol. Chem., 271, 13401-13406.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.2 expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.8.2 C30A removal of the 6-S-cysteinyl-FMN bond, diminishes limiting rate for the first of three observed kinetic phases Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.8.2 Fe 4Fe-4S-center Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.2 diethylmethylamine + H2O + electron acceptor Methylophilus methylotrophus slow substrate, triphasic reaction kinetic diethylamine + formaldehyde + reduced electron acceptor
-
 ?
1.5.8.2 diethylmethylamine + H2O + electron acceptor Methylophilus methylotrophus W3A1 slow substrate, triphasic reaction kinetic diethylamine + formaldehyde + reduced electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, recombinant enzyme Methylophilus methylotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 diethylmethylamine + H2O + electron acceptor slow substrate, triphasic reaction kinetic Methylophilus methylotrophus diethylamine + formaldehyde + reduced electron acceptor
-
 ?
1.5.8.2 diethylmethylamine + H2O + electron acceptor slow substrate, triphasic reaction kinetic Methylophilus methylotrophus W3A1 diethylamine + formaldehyde + reduced electron acceptor
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.2 FMN covalently bound to Cys30 Methylophilus methylotrophus