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Literature summary extracted from

  • Haeuser, I.; Dehesh, K.; Apel, K.
    The proteolytic degradation in vitro of the NADPH-protochlorophyllide oxidoreductase of barley (Hordeum vulgare L.) (1984), Arch. Biochem. Biophys., 228, 577-586.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.33 protochlorophyllide and NADPH enhances the stability of the enzyme Hordeum vulgare
1.3.1.33 rapid breakdown of the enzyme protein in vitro, caused by a membrane-bound proteolytic activity Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.33 PMSF
-
 Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.33 plastid plastid membrane Hordeum vulgare 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.33 36000
-
-
 Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH Hordeum vulgare
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH Avena sativa
-
 chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Avena sativa
-
 oat
-
1.3.1.33 Hordeum vulgare
-
 barley
-
1.3.1.33 Hordeum vulgare
-
 L cv. carina
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.33 leaf
-
 Hordeum vulgare
-
1.3.1.33 leaf
-
 Avena sativa
-
1.3.1.33 seedling
-
 Hordeum vulgare
-
1.3.1.33 seedling
-
 Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH
-
 Hordeum vulgare chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH
-
 Avena sativa chlorophyllide + NADP+
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.33 10 60 linear drop of the relative concentration of the enzyme protein up to 30°C Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.33 6 8.5
-
 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH no reaction with NADH Hordeum vulgare
1.3.1.33 NADPH no reaction with NADH Avena sativa
1.3.1.33 NADPH stringent requirement Hordeum vulgare