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Literature summary extracted from

  • Oliver, R.P.; Griffiths, W.T.
    Covalent labelling of the NADPH: protochlorophyllide oxidoreductase from etioplast membranes with [3H]N-phenylmaleimide (1981), Biochem. J., 195, 93-101.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.3.1.33 active enzyme is protected by NADPH, protochlorophyllide and glycerol Phaseolus vulgaris
1.3.1.33 active enzyme is protected by NADPH, protochlorophyllide and glycerol Hordeum vulgare
1.3.1.33 active enzyme is protected by NADPH, protochlorophyllide and glycerol Avena sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.33 N-ethylmaleimide
-
Avena sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.33 etioplast
-
Phaseolus vulgaris 9513
-
1.3.1.33 etioplast
-
Hordeum vulgare 9513
-
1.3.1.33 etioplast
-
Avena sativa 9513
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.33 34000
-
-
Avena sativa
1.3.1.33 34000
-
2 related peptides of 34000/35000 Phaseolus vulgaris
1.3.1.33 35000
-
-
Hordeum vulgare
1.3.1.33 35000
-
2 related peptides of 34000/35000 Phaseolus vulgaris
1.3.1.33 35000
-
SDS-PAGE shows 2 closely related peptides of 35000 and 37000 Avena sativa
1.3.1.33 37000
-
-
Hordeum vulgare
1.3.1.33 37000
-
gel filtration, doublet of 2 peptides of 35000 and 37000 co-purified Avena sativa
1.3.1.33 38000
-
-
Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH Phaseolus vulgaris
-
chlorophyllide + NADP+
-
?
1.3.1.33 protochlorophyllide + NADPH Hordeum vulgare
-
chlorophyllide + NADP+
-
?
1.3.1.33 protochlorophyllide + NADPH Avena sativa
-
chlorophyllide + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Avena sativa
-
oat
-
1.3.1.33 Avena sativa
-
L., var. Pennal
-
1.3.1.33 Hordeum vulgare
-
barley
-
1.3.1.33 Hordeum vulgare
-
L., cultivar Proctor
-
1.3.1.33 Phaseolus vulgaris
-
runner bean, L., cultivar Streamline
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH
-
Phaseolus vulgaris chlorophyllide + NADP+
-
?
1.3.1.33 protochlorophyllide + NADPH
-
Hordeum vulgare chlorophyllide + NADP+
-
?
1.3.1.33 protochlorophyllide + NADPH
-
Avena sativa chlorophyllide + NADP+
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH no reaction with NADH Phaseolus vulgaris
1.3.1.33 NADPH no reaction with NADH Hordeum vulgare
1.3.1.33 NADPH no reaction with NADH Avena sativa