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Literature summary extracted from

  • Verpoorte, J.A.
    Purification of two beta-N-acetyl-D-glucosaminidases from beef spleen (1972), J. Biol. Chem., 247, 4787-4793.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 bovine serum albumin
-
Bos taurus

General Stability

EC Number General Stability Organism
3.2.1.52 unstable during chromatography and dialysis Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+
-
Bos taurus
3.2.1.52 Fe2+
-
Bos taurus
3.2.1.52 Fe3+
-
Bos taurus
3.2.1.52 Hg2+
-
Bos taurus
3.2.1.52 p-chloromercuribenzoate
-
Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.08
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form B, pH 4.55 Bos taurus
3.2.1.52 0.15
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside form A, pH 4.55 Bos taurus
3.2.1.52 0.32
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form B, pH 5.5 Bos taurus
3.2.1.52 0.38
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form A, pH 5.5 Bos taurus
3.2.1.52 0.85
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form B, pH 4.5 Bos taurus
3.2.1.52 0.87
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form A, pH 4.55 Bos taurus
3.2.1.52 1.96
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form A, pH 3.55 Bos taurus
3.2.1.52 2.38
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside form B, pH 3.55 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 additional information
-
-
Bos taurus
3.2.1.52 140000
-
sedimentation equilibrium Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
N-acetyl-D-hexosamines
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.52 dithiothreitol 2 mM cause total inhibition Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein neutral sugar Bos taurus
3.2.1.52 glycoprotein glucosaminylation Bos taurus
3.2.1.52 glycoprotein sialylation Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 A form: 310fold, B form: 260fold, to homogeneity Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 spleen
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 18.1
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form B Bos taurus
3.2.1.52 21.1
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form A Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, crude extract Bos taurus
3.2.1.52 2°C, in (NH4)2SO4, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Bos taurus 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
Bos taurus N-acetyl-D-hexosamines
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Bos taurus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 More
-
Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 37
-
at pH 4.5, stable for 15 min Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4.5
-
at 37°C, stable for 15 min Bos taurus