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Literature summary extracted from
- An exo-beta-N-acetylglucosaminidase from Bacillus subtilis B. Extraction and purification (1972), Biochim. Biophys. Acta, 289, 174-186.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.52 | Ca2+ | 1 M and more cause 100% inhibition | Bacillus sp. (in: Bacteria) |  |
| 3.2.1.52 | Mg2+ | 0.5 M causes 78% inhibition, 1 M causes 82% inhibition, 2 M and more cause 100% inhibition | Bacillus sp. (in: Bacteria) | |
| 3.2.1.52 | NH4+ | 20% inhibition with 2 M, 5% inhibition with 2 M | Bacillus sp. (in: Bacteria) | |
| 3.2.1.52 | Urea | 19%, 33% and 40% inhibition with 4, 6 and 8 M | Bacillus sp. (in: Bacteria) | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.52 | extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | Bacillus sp. (in: Bacteria) | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | Bacillus sp. (in: Bacteria) | - |
B. subtilis | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.52 | 7000fold to homogeneity | Bacillus sp. (in: Bacteria) |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | culture filtrate | - |
Bacillus sp. (in: Bacteria) | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 23.3 | - |
substrate: p-nirophenyl-2-acetamido-2-deoxyglucose | Bacillus sp. (in: Bacteria) |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.52 | -20°C, crude enzyme, stable | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | - |
Bacillus sp. (in: Bacteria) | N-acetyl-D-hexosamines | - |
? | |
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Release 2023.1 (January 2023)
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