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Literature summary extracted from

  • Khar, A.; Anand, S.R.
    Studies on the glycosidases of semen. Purification and properties of beta-N-acetylglucosaminidase from bull sperm (1977), Biochim. Biophys. Acta, 483, 141-151.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 cysteine
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 acetate
-
 Bos taurus
3.2.1.52 Ag+
-
 Bos taurus
3.2.1.52 galactonolactone
-
 Bos taurus
3.2.1.52 galactosamine
-
 Bos taurus
3.2.1.52 gluconolactone
-
 Bos taurus
3.2.1.52 glucosamine
-
 Bos taurus
3.2.1.52 Hg2+
-
 Bos taurus
3.2.1.52 iodoacetamide
-
 Bos taurus
3.2.1.52 N-acetyl-D-galactosamine
-
 Bos taurus
3.2.1.52 N-acetyl-D-glucosamine
-
 Bos taurus
3.2.1.52 p-chloromercuribenzoate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.27
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Bos taurus
3.2.1.52 0.52
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 acrosome
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 13400
-
 1 * 53000 + 1 * 13400, SDS-PAGE Bos taurus
3.2.1.52 53000
-
 1 * 53000 + 1 * 13400, SDS-PAGE Bos taurus
3.2.1.52 190000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
 N-acetyl-D-hexosamines
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.52 2-mercaptoethanol denaturation in subunits Bos taurus
3.2.1.52 guanidine-HCl denaturation in subunits Bos taurus
3.2.1.52 urea denaturation in subunits Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 135fold to homogeneity Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose seperate active sites for glucosaminidase and galactosaminidase activities Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 semen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 0.63
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 alpha1-acid glycoprotein + H2O
-
 Bos taurus ?
-
 ?
3.2.1.52 chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-4 Bos taurus N-acetyl-D-glucosamine
-
 ?
3.2.1.52 kappa-casein glycopeptide + H2O
-
 Bos taurus ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Bos taurus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 ovalbumin glycopeptides + H2O
-
 Bos taurus ?
-
 ?
3.2.1.52 ovomucoid glycopeptide + H2O
-
 Bos taurus ?
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer 1 * 53000 + 1 * 13400, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 37
-
 stable for 60 min at pH 4.5 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 3.4
-
 N-acetylgalactosamnidase activity Bos taurus
3.2.1.52 4.5
-
 N-acetylglucosamnidase activity Bos taurus