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Literature summary extracted from

  • Jones, C.S.; Kosman, D.J.
    Purification, properties, kinetics, and mechanism of beta-N-acetylglucosaminidase from Aspergillus niger (1980), J. Biol. Chem., 255, 11861-11869.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ 100 mM Aspergillus niger
3.2.1.52 Ca2+ 100 mM Aspergillus niger
3.2.1.52 Cu2+ 100 mM Aspergillus niger
3.2.1.52 Hg2+ 100 mM Aspergillus niger
3.2.1.52 N-acetyl-beta-D-glucosaminolactone
-
 Aspergillus niger
3.2.1.52 N-acetyl-D-glucosamine
-
 Aspergillus niger
3.2.1.52 p-thiophenyl-1-thio-N-acetyl-beta-D-glucosaminide
-
 Aspergillus niger
3.2.1.52 Zn2+ 100 mM Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.27
-
 2,4-dinitrophenyl-N-acetyl-beta-D-glucosaminide
-
 Aspergillus niger
3.2.1.52 0.34
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Aspergillus niger
3.2.1.52 0.86
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Aspergillus niger
3.2.1.52 1.3
-
 p-nitrophenyl-thio-beta-N-acetyl-D-glucosaminide
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 149000
-
 gel filtration and amino acid composition Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Aspergillus niger
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein mannosylation Aspergillus niger
3.2.1.52 glycoprotein galactosylation Aspergillus niger
3.2.1.52 glycoprotein N-acetyl-glucosaminylation Aspergillus niger
3.2.1.52 glycoprotein fucosylation Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 2 isozymes, 153fold to homogeneity Aspergillus niger

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Aspergillus niger
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 additional information
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 2,4-dinitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Aspergillus niger 2,4-dinitrophenol + N-acetyl-beta-D-glucosaminide
-
 ?
3.2.1.52 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Aspergillus niger N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Aspergillus niger 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Aspergillus niger 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-thio-beta-N-acetyl-D-glucosaminide + H2O
-
 Aspergillus niger 4-nitrophenol + thio-beta-N-acetyl-D-glucosaminide
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 additional information
-
 additional information
-
 Aspergillus niger
3.2.1.52 0.0167
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside in D2O, pD 7.0 Aspergillus niger
3.2.1.52 0.0212
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside in D2O, pD 4.6 Aspergillus niger
3.2.1.52 2.83
-
 p-nitrophenyl-thio-beta-N-acetyl-D-glucosaminide
-
 Aspergillus niger
3.2.1.52 38.3
-
 2,4-dinitrophenyl-N-acetyl-beta-D-glucosaminide
-
 Aspergillus niger
3.2.1.52 110
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Aspergillus niger
3.2.1.52 187
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 additional information
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 additional information
-
-
 Aspergillus niger