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Literature summary extracted from

  • Garcia-Alonso, J.; Reglero, A.; Cabezas, J.A.
    Purification and properties of beta-N-acetylhexosaminidase A from pig brain (1990), Int. J. Biochem., 22, 645-651.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 2-acetamido-2-deoxy-D-galactono(1-4)lactone with 10 mM 75% inhibition Sus scrofa
3.2.1.52 D-mannose with 10 mM 30% inhibition Sus scrofa
3.2.1.52 mannosamine with 10 mM 80% inhibition Sus scrofa
3.2.1.52 methyl-alpha-D-mannoside concentration of 10 mM causes 25% inhibition Sus scrofa
3.2.1.52 additional information
-
 Sus scrofa
3.2.1.52 N-acetyl-beta-D-glucosaminolactone with 10 mM 75% inhibition Sus scrofa
3.2.1.52 N-acetyl-D-galactosamine concentration of 10 mM causes 80% inhibition Sus scrofa
3.2.1.52 N-acetyl-D-glucosamine concentration of 10 mM causes 60% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.2
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Sus scrofa
3.2.1.52 1.8
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 160000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Sus scrofa
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 form A: 1300-1500fold to homogeneity Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Sus scrofa
a
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 5.1
-
 N-acetylgalactosaminidase activity Sus scrofa
3.2.1.52 46.7
-
 N-acetylglucosaminidase activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Sus scrofa N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Sus scrofa N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Sus scrofa 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Sus scrofa 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.1 4.3 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside or -glucosaminide Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4 7.5 stable for 2 hr at 37°C Sus scrofa