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Literature summary extracted from

  • Li, S.C.; Li, Y.T.
    Studies on the glycosidases of jack bean meal, III. Crystallisation and properties of beta-N-acetylhexosaminidase (1970), J. Biol. Chem., 245, 5153-5160.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.52
-
 Canavalia ensiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 acetamide with 10 mM 70% of activity remains Canavalia ensiformis
3.2.1.52 Ag+ complete inactivation with 0.1 mM Canavalia ensiformis
3.2.1.52 Ba2+ with 10 mM 85% of activity remains Canavalia ensiformis
3.2.1.52 Cu2+ with 10 mM 78% of activity remains Canavalia ensiformis
3.2.1.52 Fe3+ with 10 mM 5% of activity remains Canavalia ensiformis
3.2.1.52 Hg2+ complete inactivation with 0.1 mM Canavalia ensiformis
3.2.1.52 Mg2+ with 10 mM 85% of activity remains Canavalia ensiformis
3.2.1.52 Mn2+ with 10 mM 82% of activity remains Canavalia ensiformis
3.2.1.52 N-acetyl-beta-D-glucosaminolactone with 0.002 mM 65% of activity remains Canavalia ensiformis
3.2.1.52 N-acetyl-D-galactosamine with 10 mM 20% of activity remains Canavalia ensiformis
3.2.1.52 N-acetyl-D-glucosamine with 10 mM 88% of activity remains Canavalia ensiformis
3.2.1.52 Sodium acetate with 10 mM 50% of activity remains Canavalia ensiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.31
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Canavalia ensiformis
3.2.1.52 0.64
-
 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 Canavalia ensiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 100000
-
 gel filtration Canavalia ensiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Canavalia ensiformis
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Canavalia ensiformis
-
 Jack bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 to homogeneity Canavalia ensiformis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Canavalia ensiformis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 seed meal Canavalia ensiformis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 101.9
-
 substrate: p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Canavalia ensiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Canavalia ensiformis 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.52 alpha1-acid glycoprotein + H2O sialic acid- and galactose-free, from human and chimpanzee Canavalia ensiformis ?
-
 ?
3.2.1.52 cyclohexyl beta-N-acetylglucosaminide + H2O
-
 Canavalia ensiformis cyclohexanol + beta-N-acetyl-D-glucosamine
-
 ?
3.2.1.52 ethyl-beta-N-acetyl-glucosaminide + H2O
-
 Canavalia ensiformis ethanol + beta-N-acetyl-glucosamine
-
 ?
3.2.1.52 globoside + H2O from human kidney Canavalia ensiformis ?
-
 ?
3.2.1.52 methyl-beta-N-acetyl-glucosaminide + H2O
-
 Canavalia ensiformis methanol + beta-N-acetyl-glucosamine
-
 ?
3.2.1.52 additional information
-
 Canavalia ensiformis ?
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Canavalia ensiformis N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Canavalia ensiformis N-acetyl-D-hexosamines
-
 ?
3.2.1.52 ovalbumin + H2O
-
 Canavalia ensiformis N-acetylglucosamine + ?
-
 ?
3.2.1.52 ovalbumin glycopeptides + H2O
-
 Canavalia ensiformis ?
-
 ?
3.2.1.52 ovomucoid + H2O
-
 Canavalia ensiformis N-acetylglucosamine + ?
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Canavalia ensiformis 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Canavalia ensiformis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 Tay-Sachs ganglioside + H2O asialo derivative Canavalia ensiformis ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 additional information
-
-
 Canavalia ensiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 3.5 4 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Canavalia ensiformis
3.2.1.52 5 6 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Canavalia ensiformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 additional information
-
-
 Canavalia ensiformis