EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.52 | dithioerythritol | presence decreases extent of inactivation by Hg2+, Ag2+, thimerosal or p-hydroxymercuribenzoate | Rattus norvegicus | |
3.2.1.52 | glutathione | presence decreases extent of inactivation by Hg2+, Ag2+, thimerosal or p-hydroxymercuribenzoate | Rattus norvegicus | |
3.2.1.52 | L-cysteine | presence decreases extent of inactivation by Hg2+, Ag2+, thimerosal or p-hydroxymercuribenzoate | Rattus norvegicus | |
3.2.1.52 | p-nitrophenyl-N-acetyl-beta-D-galactosaminide | presence at a concentration 10 times that of p-hydroxymercuribenzoate lowers loss of galactosaminidase-activity by 25.5% | Rattus norvegicus | |
3.2.1.52 | p-nitrophenyl-N-acetyl-beta-D-glucosaminide | presence at a concentration 50 times that of Hg2+, Ag+, thimerosal or p-hydroxymercuribenzoate reduces loss of glucosaminidase-activity by 20.5% | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.52 | Ag+ | completely inactivates all 3 enzyme forms in colonic mucosa | Rattus norvegicus | |
3.2.1.52 | dithioerythritol | concentration of 2.5 mM decreases activity of the 3 enzyme forms in colonic mucosa by 20% at pH 4.2 and by 25% at pH 7.0, similar results with the 2 forms from colon tumors | Rattus norvegicus | |
3.2.1.52 | Hg2+ | completely inactivates all 3 enzyme forms in colonic mucosa | Rattus norvegicus | |
3.2.1.52 | mannosamine | - |
Rattus norvegicus | |
3.2.1.52 | N-acetyl-beta-D-galactosaminolactone | - |
Rattus norvegicus | |
3.2.1.52 | N-acetyl-beta-D-glucosaminolactone | - |
Rattus norvegicus | |
3.2.1.52 | N-acetyl-D-galactosamine | - |
Rattus norvegicus | |
3.2.1.52 | N-acetyl-D-glucosamine | - |
Rattus norvegicus | |
3.2.1.52 | p-hydroxymercuribenzoate | forms A and I retain 50% of glucosaminidase-activity if treated with 0.005 mM at pH 4.2, 5.6 or 7.0 for 15 min, glucosaminidase-activity of form B is stable at pH 4.2 and 7.0 in presence of 0.005 mM and inactivated by less than 5% at pH 5.6, galactosaminidase-activity is decreased by 50% if form A and I was treated with 0.1 mM at pH 4.2, 5.6 or 7.0, galactosaminidase-activity of form B is stable | Rattus norvegicus | |
3.2.1.52 | thimerosal | inhibits activity in mucosal and tumor enzymes, 50% of activity remains after 1 h if form A is treated with 0.20 mM, form B with 1.75 mM and form I with 0.65 mM | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.52 | 0.064 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | colonic tumor, form B | Rattus norvegicus | |
3.2.1.52 | 0.095 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | colonic mucosa, form B | Rattus norvegicus | |
3.2.1.52 | 0.2 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | colonic mucosa, form I | Rattus norvegicus | |
3.2.1.52 | 0.26 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | colonic tumor, form B | Rattus norvegicus | |
3.2.1.52 | 0.31 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | colonic mucosa, form B | Rattus norvegicus | |
3.2.1.52 | 0.33 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | colonic mucosa: form A | Rattus norvegicus | |
3.2.1.52 | 0.33 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | colonic tumor: form A | Rattus norvegicus | |
3.2.1.52 | 0.53 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | colonic mucosa, form I | Rattus norvegicus | |
3.2.1.52 | 0.73 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | colonic tumor, form A | Rattus norvegicus | |
3.2.1.52 | 0.76 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | colonic mucosa, form A | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | Rattus norvegicus | - |
N-acetyl-D-hexosamines | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.52 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.52 | colonic mucosa: form A 4800fold, form B 5110fold, form I 4100fold, colonic tumors: form A 5090fold, form B 5620fold | Rattus norvegicus |
3.2.1.52 | N-acetylhexosaminidase: 63fold | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.52 | (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | seperate active sites for glucosaminidase and galactosaminidase activities | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.52 | colonic carcinoma cell | - |
Rattus norvegicus | - |
3.2.1.52 | colonic mucosa | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 0.75 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic mucosa, form I | Rattus norvegicus |
3.2.1.52 | 0.88 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic mucosa, form A | Rattus norvegicus |
3.2.1.52 | 0.94 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic mucosa, form B | Rattus norvegicus |
3.2.1.52 | 1.78 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic tumor, form A | Rattus norvegicus |
3.2.1.52 | 1.97 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic tumor, form B | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | - |
Rattus norvegicus | N-acetyl-D-hexosamines | - |
? | |
3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Rattus norvegicus | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Rattus norvegicus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
3.2.1.52 | phenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Rattus norvegicus | phenol + N-acetyl-beta-D-glucosaminide | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 50 | - |
mucosal enzymes, relatively slow inactivation, tumor B-form shows greater stability than mucosal B-form | Rattus norvegicus |
3.2.1.52 | 55 | - |
mucosal enzymes, rapid and almost complete inactivation after 5 min | Rattus norvegicus |
3.2.1.52 | 60 | - |
mucosal enzymes, rapid and almost complete inactivation after 5 min | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 4.2 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Rattus norvegicus |