Literature summary extracted from
Okada, G.
Enzymatic studies on a cellulase system of Trichoderma viride. II. Purification and properties of two cellulases (1975), J. Biochem., 77, 33-42.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.4 |
Ag+ |
1 mM, partial inhibition |
Trichoderma viride |
|
3.2.1.4 |
Cu2+ |
1 mM, partial inhibition |
Trichoderma viride |
|
3.2.1.4 |
Hg2+ |
1 mM, complete inhibition |
Trichoderma viride |
|
3.2.1.4 |
KMnO4 |
0.1 mM, complete inhibition of cellulase II-A |
Trichoderma viride |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.4 |
30000 |
- |
cellulase II-A, gel filtration |
Trichoderma viride |
3.2.1.4 |
43000 |
- |
cellulase II-B, gel filtration |
Trichoderma viride |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.4 |
Trichoderma viride |
- |
cellulase II-A and II-B |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.2.1.4 |
glycoprotein |
cellulase II-A contains 12% carbohydrate, cellulase II-B contains 14% carbohydrate |
Trichoderma viride |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.4 |
cellulase II-A and II-B |
Trichoderma viride |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.4 |
50 |
- |
cellulase II-B |
Trichoderma viride |
3.2.1.4 |
60 |
- |
cellulase IIa |
Trichoderma viride |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.4 |
45 |
- |
2 h, cellulase II-B is stable over the pH-range 4.5-6.0, cellulase II-A is unstable, except at pH 5.5-6.0 |
Trichoderma viride |
3.2.1.4 |
100 |
- |
10 min, cellulase II-A retains 27% of the original activity, cellulase II-B retains 41% of the original activity |
Trichoderma viride |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.4 |
4.5 |
5 |
- |
Trichoderma viride |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.2.1.4 |
4.5 |
6 |
45°C, 2 h, cellulase II-B is stable |
Trichoderma viride |
3.2.1.4 |
5.5 |
6 |
5°C, 2 h, cellulase II-A is stable |
Trichoderma viride |