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Literature summary extracted from

  • Wood, T.M.; McCrae, S.I.
    The cellulase of Trichoderma koningii. Purification and properties of some endoglucanase components with special reference to their action on cellulose when acting alone and in synergism with the cellobiohydrolase (1978), Biochem. J., 171, 61-72.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 cellobiose 0.29 mM, stimulation Trichoderma koningii
3.2.1.4 methylcellulose 0.1%, 20% stimulation of carboxymethylcellulase activity of enzyme E1 Trichoderma koningii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 cellobiose inhibition of endoglucanase E3a, E3b and E4 Trichoderma koningii
3.2.1.4 glucosamine inhibition of CM-cellulase activity of enzyme E1, E3a, E3b and E4 Trichoderma koningii
3.2.1.4 glucose 29 mM, 12% inhibition of endoglucanase E4, no inhibition of endoglucanase E1 and E3a Trichoderma koningii
3.2.1.4 methylcellulose poor inhibitor of carboxymethylcellulase activity of enzyme form E3a, E3b and E4 Trichoderma koningii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 13000
-
 endoglucanase E1, gel filtration Trichoderma koningii
3.2.1.4 31000
-
 endoglucanase E4, gel filtration Trichoderma koningii
3.2.1.4 48000
-
 enoglucanase E3a, gel filtration Trichoderma koningii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Trichoderma koningii
-
-
-
3.2.1.176 Trichoderma koningii
-
 CBH 1, CBH 1a
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 endoglucanase E1, E3a and E4 Trichoderma koningii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Trichoderma koningii additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Trichoderma koningii additional information
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Trichoderma koningii 2 cellobiose
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Trichoderma koningii ?
-
 ?
3.2.1.4 additional information hydrolysis of cotton Trichoderma koningii ?
-
 ?
3.2.1.176 cellulose + H2O
-
 Trichoderma koningii cellobiose + D-glucose + cellotriose different combinations and proportions of products depending on organism ?
3.2.1.176 additional information
-
 Trichoderma koningii ?
-
 ?