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Literature summary extracted from

  • Okada, G.
    Cellulase of Aspergillus niger (1988), Methods Enzymol., 160, 259-264.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ag+ 1 mM, 75% inhibition Aspergillus niger
3.2.1.4 Fe2+ 1 mM, 55% inhibition Aspergillus niger
3.2.1.4 Hg2+ 1 mM, 67% inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 31000
-
 x * 31000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.4 cellulose + H2O Aspergillus niger the enzyme plays an important role in maintaining the carbon balance in nature cellooligosaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 116.8
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
3.2.1.4 4°C, pH 5.0-8.0, 24 h, stable Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Aspergillus niger additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Aspergillus niger additional information
-
 ?
3.2.1.4 cellulose + H2O the enzyme plays an important role in maintaining the carbon balance in nature Aspergillus niger cellooligosaccharide
-
 ?
3.2.1.4 additional information no activity with p-nitrophenyl-beta-D-glucoside Aspergillus niger ?
-
 ?
3.2.1.4 additional information no activity with cellobiose Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 31000, SDS-PAGE Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 70
-
 10 min, 50% loss of activity Aspergillus niger
3.2.1.4 80
-
 10 min, complete inactivation Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4
-
 hydrolysis of carboxymethylcellulose Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 8 4°C, 24 h, stable Aspergillus niger