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Literature summary extracted from

  • Robson, L.M.; Chambliss, G.H.
    Cellulases of bacterial origin (1989), Enzyme Microb. Technol., 11, 626-644.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 endoglucanase Y Dickeya chrysanthemi
3.2.1.4 expression in Escherichia coli Ruminococcus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ca2+ slight inhibition of endoglucanase E2 activity Thermobifida fusca
3.2.1.4 cellobiose
-
 Acetivibrio cellulolyticus
3.2.1.4 cellobiose
-
 Acetivibrio thermocellus
3.2.1.4 cellobiose 80 mM, 14% loss of activity, endoglucanase Z Dickeya chrysanthemi
3.2.1.4 cellobiose
-
 Thermobifida fusca
3.2.1.4 glucose 162 mM, 23% loss of activity, endoglucanase Z Dickeya chrysanthemi
3.2.1.4 Mg2+ slight inhibition of endoglucanase E2 activity Thermobifida fusca

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular endoglucanase C2 and C3 Acetivibrio cellulolyticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+
-
 Acetivibrio thermocellus
3.2.1.4 Ca2+
-
 Acetivibrio cellulolyticus
3.2.1.4 Ca2+ stimulates activity of endoglucanase E1 Thermobifida fusca
3.2.1.4 Mg2+
-
 Acetivibrio thermocellus
3.2.1.4 Mg2+
-
 Acetivibrio cellulolyticus
3.2.1.4 Mg2+ stimulates activity of endoglucanase E1 Thermobifida fusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 35000
-
 x * 35000, endoglucanase Y, SDS-PAGE Dickeya chrysanthemi
3.2.1.4 40000
-
 x * 40000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 45000
-
 endoglucanase Z Dickeya chrysanthemi
3.2.1.4 56000
-
 x * 56000, enzyme form EGA, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 65000
-
 x * 65000, enzyme form EGD, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 66000
-
 x * 66000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 94000
-
 1 * 94000, endoglucanase E1, SDS-PAGE Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Acetivibrio cellulolyticus
-
-
-
3.2.1.4 Acetivibrio thermocellus
-
 enzyme form EGA, EGB, EGC and EGD
-
3.2.1.4 Bacillus sp. (in: Bacteria)
-
 No. 1139
-
3.2.1.4 Bacillus sp. (in: Bacteria)
-
 N-4
-
3.2.1.4 Bacillus sp. (in: Bacteria) N-4
-
 N-4
-
3.2.1.4 Bacillus sp. (in: Bacteria) No. 1139
-
 No. 1139
-
3.2.1.4 Bacillus subtilis
-
 DLG, N-24, or PAP115
-
3.2.1.4 Cellulomonas fimi
-
 endoglucanase A and B
-
3.2.1.4 Dickeya chrysanthemi
-
 endoglucanase Z and Y
-
3.2.1.4 Ruminococcus albus
-
 F40
-
3.2.1.4 Ruminococcus albus F40
-
 F40
-
3.2.1.4 Thermobifida fusca
-
 endoglucanase E1, E2, and E5
-
3.2.1.4 Thermomonospora curvata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein
-
 Acetivibrio thermocellus
3.2.1.4 glycoprotein endoglucanase E2 contains 25% carbohydrate Thermobifida fusca
3.2.1.4 no modification
-
 Acetivibrio thermocellus
3.2.1.4 no modification endoglucanase E1 may not be a glycoprotein Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Bacillus subtilis additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Ruminococcus albus additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Dickeya chrysanthemi additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Acetivibrio thermocellus additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Cellulomonas fimi additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Acetivibrio cellulolyticus additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O endoglucanase E1 exhibits higher activity Thermobifida fusca additional information endoglucanase E2 produces glucose, cellobiose, cellotriose and higher oligosaccharides. The major end product produced by endoglucanase E1 is cellobiose and small amounts of glucose ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) No. 1139 additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) N-4 additional information
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Ruminococcus albus F40 additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Ruminococcus albus additional information
-
 ?
3.2.1.4 cellopentaose + H2O enzyme form EGB and EGC Acetivibrio thermocellus additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Ruminococcus albus F40 additional information
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Bacillus sp. (in: Bacteria) 2 cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Ruminococcus albus 2 cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 Thermobifida fusca 2 cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O enzyme form EGB and EGC Acetivibrio thermocellus 2 cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Bacillus sp. (in: Bacteria) No. 1139 2 cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Bacillus sp. (in: Bacteria) N-4 2 cellobiose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Ruminococcus albus F40 2 cellobiose
-
 ?
3.2.1.4 cellotriose + H2O
-
 Bacillus sp. (in: Bacteria) D-glucose + cellobiose
-
 ?
3.2.1.4 cellotriose + H2O enzyme form EGC Acetivibrio thermocellus D-glucose + cellobiose
-
 ?
3.2.1.4 cellotriose + H2O effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 Thermobifida fusca D-glucose + cellobiose
-
 ?
3.2.1.4 cellotriose + H2O
-
 Bacillus sp. (in: Bacteria) No. 1139 D-glucose + cellobiose
-
 ?
3.2.1.4 cellotriose + H2O
-
 Bacillus sp. (in: Bacteria) N-4 D-glucose + cellobiose
-
 ?
3.2.1.4 cellulose + H2O
-
 Acetivibrio cellulolyticus ?
-
 ?
3.2.1.4 cellulose + H2O endoglucanase E1 exhibits higher activity Thermobifida fusca ?
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Ruminococcus albus ?
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Thermobifida fusca ?
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Acetivibrio thermocellus ?
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Dickeya chrysanthemi ? major end product are cellobiose and cellotriose, endoglucanase Z ?
3.2.1.4 cellulose + H2O enzyme form EGA and EGD Acetivibrio thermocellus ?
-
 ?
3.2.1.4 cellulose + H2O acid-swollen Ruminococcus albus F40 ?
-
 ?
3.2.1.4 lichenan + H2O enzyme form EGD Acetivibrio thermocellus ?
-
 ?
3.2.1.4 lichenan + H2O endoglucanase A Cellulomonas fimi ?
-
 ?
3.2.1.4 xylan + H2O
-
 Thermobifida fusca additional information
-
 ?
3.2.1.4 xylan + H2O endoglucanase B Cellulomonas fimi additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 56000, enzyme form EGA, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 ? 1 * 94000, endoglucanase E1, SDS-PAGE Thermobifida fusca
3.2.1.4 ? x * 40000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 ? x * 66000, enzyme form EGB, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 ? x * 35000, endoglucanase Y, SDS-PAGE Dickeya chrysanthemi
3.2.1.4 ? x * 65000, enzyme form EGD, SDS-PAGE Acetivibrio thermocellus
3.2.1.4 More enzyme form C2, 33000 Da, is likely to by a trimer of endoglucanase C3, 104000 Da Acetivibrio cellulolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 52
-
 hydrolysis of carboxymethylcellulose, endoglucanase Z Dickeya chrysanthemi
3.2.1.4 58
-
 endoglucanase E2 Thermobifida fusca
3.2.1.4 60
-
-
 Bacillus subtilis
3.2.1.4 60
-
 enzyme form EGD Acetivibrio thermocellus
3.2.1.4 74
-
 endoglucanase E1 Thermobifida fusca
3.2.1.4 75
-
 enzyme form EGA Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.4 50
-
 stable up to Bacillus subtilis
3.2.1.4 60
-
-
 Bacillus sp. (in: Bacteria)
3.2.1.4 60
-
 enzyme form EGA, stable up to Acetivibrio thermocellus
3.2.1.4 65
-
-
 Bacillus subtilis
3.2.1.4 65
-
 enzyme form EGC, stable up to Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5 6
-
 Bacillus subtilis
3.2.1.4 5.5
-
-
 Bacillus subtilis
3.2.1.4 5.5
-
 endoglucanase Y Dickeya chrysanthemi
3.2.1.4 5.5 6.5 enzyme form EGA Acetivibrio thermocellus
3.2.1.4 6
-
 endoglucanase E1 and E2 Thermobifida fusca
3.2.1.4 6
-
 enzyme form EGC and EGD Acetivibrio thermocellus
3.2.1.4 6.2 7.5 hydrolysis of carboxymethylcellulose, endoglucanase Z Dickeya chrysanthemi