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Literature summary extracted from

  • Sakai, K.; Yokota, A.; Kurokawa, H.; Wakayama, M.; Moriguchi, M.
    Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost (1998), Appl. Environ. Microbiol., 64, 3397-3402.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ag+
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 diacetylchitobiose
-
 Bacillus sp. (in: Bacteria)
3.2.1.14 Hg2+
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+ all isoenzymes stimulated by Ca2+ Bacillus sp. (in: Bacteria)
3.2.1.14 Mg2+ all isoenzymes stimulated by Mg2+ Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 22000
-
 isoenzyme III, gel filtration Bacillus sp. (in: Bacteria)
3.2.1.14 44000
-
 isoenzyme II, gel filtration Bacillus sp. (in: Bacteria)
3.2.1.14 53000
-
 isoenzyme III, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.14 62000
-
 isoenzyme II, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.14 68000
-
 isoenzyme I, gel filtration Bacillus sp. (in: Bacteria)
3.2.1.14 71000
-
 isoenzyme I, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.14 Bacillus sp. (in: Bacteria) MH-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 isoenzyme I, 20fold, isoenzyme II 18fold, isoenzyme II 24fold Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 culture filtrate
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.472
-
 isoenzyme II Bacillus sp. (in: Bacteria)
3.2.1.14 0.547
-
 isoenzyme I Bacillus sp. (in: Bacteria)
3.2.1.14 0.641
-
 isoenzyme III Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal chitin Bacillus sp. (in: Bacteria) N,N-diacetylchitobiose + N-acetylglucosamine chitobiose and chitotriose main products ?
3.2.1.14 chitin + H2O colloidal chitin Bacillus sp. (in: Bacteria) MH-1 N,N-diacetylchitobiose + N-acetylglucosamine chitobiose and chitotriose main products ?
3.2.1.14 chitohexaose + H2O
-
 Bacillus sp. (in: Bacteria) chitobiose + chitotriose
-
 ?
3.2.1.14 chitohexaose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 chitobiose + chitotriose
-
 ?
3.2.1.14 chitopentaose + H2O
-
 Bacillus sp. (in: Bacteria) chitobiose + chitotriose
-
 ?
3.2.1.14 chitopentaose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 chitobiose + chitotriose
-
 ?
3.2.1.14 chitotetraose + H2O
-
 Bacillus sp. (in: Bacteria) N-acetylglucosamine + chitobiose + chitotriose
-
 ?
3.2.1.14 chitotetraose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 N-acetylglucosamine + chitobiose + chitotriose
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
 Bacillus sp. (in: Bacteria) N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Bacillus sp. (in: Bacteria) N,N'-diacetylchitobiose + p-nitrophenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 65
-
 isoenzyme II Bacillus sp. (in: Bacteria)
3.2.1.14 75
-
 isoenzyme I and II Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 80
-
 isoenzyme II, completely inactivated Bacillus sp. (in: Bacteria)
3.2.1.14 85
-
 isoenzyme III, completely inactivated Bacillus sp. (in: Bacteria)
3.2.1.14 90
-
 isoenzyme I, completely inactivated Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
 isoenzyme II Bacillus sp. (in: Bacteria)
3.2.1.14 5.5
-
 isoenzyme III Bacillus sp. (in: Bacteria)
3.2.1.14 6.5
-
 isoenzyme I Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 9 isoenzyme III Bacillus sp. (in: Bacteria)
3.2.1.14 4.5 9 isoenzyme II Bacillus sp. (in: Bacteria)
3.2.1.14 6 9 isoenzyme I Bacillus sp. (in: Bacteria)