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Literature summary extracted from
- Chitinase in goat serum. Preliminary purification and characterization (1974), Eur. J. Biochem., 46, 367-376.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 59000 | 60000 | gel filtration | Capra hircus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Bos taurus | - |
- |
- |
| 3.2.1.14 | Capra hircus | - |
- |
- |
| 3.2.1.14 | Gallus gallus | - |
- |
- |
| 3.2.1.14 | Mus musculus | - |
- |
- |
| 3.2.1.14 | Ovis aries | - |
- |
- |
| 3.2.1.14 | Rattus norvegicus | - |
- |
- |
| 3.2.1.14 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | 100fold | Capra hircus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | abomasum | - |
Capra hircus | - |
| 3.2.1.14 | abomasum | mucosa, mucus and wall | Bos taurus | - |
| 3.2.1.14 | kidney | - |
Bos taurus | - |
| 3.2.1.14 | leukocyte | - |
Bos taurus | - |
| 3.2.1.14 | leukocyte | - |
Capra hircus | - |
| 3.2.1.14 | pancreas | - |
Bos taurus | - |
| 3.2.1.14 | pancreas | - |
Capra hircus | - |
| 3.2.1.14 | serum | - |
Gallus gallus | - |
| 3.2.1.14 | serum | - |
Mus musculus | - |
| 3.2.1.14 | serum | - |
Rattus norvegicus | - |
| 3.2.1.14 | serum | - |
Sus scrofa | - |
| 3.2.1.14 | serum | - |
Bos taurus | - |
| 3.2.1.14 | serum | - |
Ovis aries | - |
| 3.2.1.14 | serum | - |
Capra hircus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | additional information | - |
- |
Bos taurus |
| 3.2.1.14 | additional information | - |
2870 Hultin Units/mg | Capra hircus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.14 | -20°C, 3 weeks, 84% activity loss | Capra hircus |
| 3.2.1.14 | 25°C, 3 weeks, no loss of activity | Capra hircus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | chitin + H2O | - |
Bos taurus | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? |  |
| 3.2.1.14 | chitin + H2O | - |
Capra hircus | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| 3.2.1.14 | glycol chitin + H2O | - |
Bos taurus | N-acetylglucosamine + ? | - |
? | |
| 3.2.1.14 | glycol chitin + H2O | - |
Capra hircus | N-acetylglucosamine + ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 60 | - |
- |
Bos taurus |
| 3.2.1.14 | 60 | - |
50% activity loss after 60 min | Capra hircus |
| 3.2.1.14 | 65 | - |
- |
Bos taurus |
| 3.2.1.14 | 65 | - |
complete inactivation after 60 min | Capra hircus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 1.65 | - |
glycol chitin | Capra hircus |
| 3.2.1.14 | 5.5 | - |
colloidal chitin | Capra hircus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | 7 | colloidal chitin as substrate | Capra hircus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 2.5 | 8 | - |
Capra hircus |
| 3.2.1.14 | 5.5 | - |
maximum stabtility at pH 5.5 | Capra hircus |
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