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Literature summary extracted from
- Chitinase from Verticillum albo-atrum (1988), Methods Enzymol., 161, 474-479.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | KCl | - |
Verticillium albo-atrum |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | 2-Acetamido-2-deoxy-D-gluconolactone | - |
Verticillium albo-atrum | |
| 3.2.1.14 | CuSO4 | - |
Verticillium albo-atrum | |
| 3.2.1.14 | EDTA | - |
Verticillium albo-atrum | |
| 3.2.1.14 | HgCl2 | - |
Verticillium albo-atrum | |
| 3.2.1.14 | KCN | - |
Verticillium albo-atrum | |
| 3.2.1.14 | LKB ampholytes | - |
Verticillium albo-atrum | |
| 3.2.1.14 | MnSO4 | - |
Verticillium albo-atrum | |
| 3.2.1.14 | ZnSO4 | - |
Verticillium albo-atrum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | additional information | - |
additional information | chitin, 97 mg dry weight per ml | Verticillium albo-atrum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.14 | extracellular | - |
Verticillium albo-atrum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | Mg2+ | - |
Verticillium albo-atrum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 64000 | - |
x * 64000, SDS-PAGE | Verticillium albo-atrum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Verticillium albo-atrum | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | culture filtrate | - |
Verticillium albo-atrum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | additional information | - |
- |
Verticillium albo-atrum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.14 | 2°C, several months, no loss of activity | Verticillium albo-atrum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | 3,4-dinitrophenyltetra-N-acetyl-beta-D-chitotetraoside + H2O | - |
Verticillium albo-atrum | ? | - |
? | |
| 3.2.1.14 | chitin + H2O | from ground crab shell | Verticillium albo-atrum | N,N-diacetylchitobiose + N-acetylglucosamine | N-acetylglucosamine only | ? | |
| 3.2.1.14 | chitosan + H2O | - |
Verticillium albo-atrum | ? | - |
? | |
| 3.2.1.14 | N,N'-diacetylchitobiose + H2O | - |
Verticillium albo-atrum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 64000, SDS-PAGE | Verticillium albo-atrum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 51 | - |
- |
Verticillium albo-atrum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 70 | - |
enzyme inactivated at 70°C | Verticillium albo-atrum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 3.7 | - |
- |
Verticillium albo-atrum |
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