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Literature summary extracted from
- Secretion, purification, and characterisation of barley alpha-amylase produced by heterologous gene expression in Aspergillus niger (1998), Appl. Microbiol. Biotechnol., 49, 385-392.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | secreted and correctly processed by Aspergillus niger | Hordeum vulgare |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | 0.38 | - |
amylose | DP 17, malt enzyme, calculated as low molecular weight products | Hordeum vulgare |  |
| 3.2.1.1 | 0.45 | - |
amylose | DP 17, recombinant enzyme, calculated as low molecular weight products | Hordeum vulgare | |
| 3.2.1.1 | 1.16 | - |
2-chloro-4-nitrophenyl beta-D-maltoheptaoside | malt enzyme | Hordeum vulgare | |
| 3.2.1.1 | 2.49 | - |
2-chloro-4-nitrophenyl beta-D-maltoheptaoside | recombinant enzyme | Hordeum vulgare | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Hordeum vulgare | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | recombinant enzyme | Hordeum vulgare |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | 2-chloro-4-nitrophenyl beta-D-maltoheptaoside + H2O | - |
Hordeum vulgare | ? | - |
? | |
| 3.2.1.1 | amylose + H2O | short chain, DP 17 | Hordeum vulgare | additional information | - |
? | |
| 3.2.1.1 | starch + H2O | soluble starch | Hordeum vulgare | additional information | - |
? | |
| 3.2.1.1 | starch + H2O | insoluble starch | Hordeum vulgare | additional information | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
additional information | - |
Hordeum vulgare | |
| 3.2.1.1 | 40.4 | - |
2-chloro-4-nitrophenyl beta-D-maltoheptaoside | malt enzyme | Hordeum vulgare | |
| 3.2.1.1 | 63.1 | - |
2-chloro-4-nitrophenyl beta-D-maltoheptaoside | recombinant enzyme | Hordeum vulgare | |
| 3.2.1.1 | 144 | - |
amylose | DP 17, malt enzyme, calculated as low molecular weight products | Hordeum vulgare | |
| 3.2.1.1 | 185 | - |
amylose | DP 17, recombinant enzyme, calculated as low molecular weight products | Hordeum vulgare | |
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