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Literature summary extracted from
- An amylase producing maltotriose from Bacillus subtilis (1985), Agric. Biol. Chem., 49, 1091-1097.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ag+ | 5 mM AgNO3, 82% inhibition | Bacillus subtilis |  |
| 3.2.1.1 | Ba2+ | 5 mM BaCl2, 28% inhibition | Bacillus subtilis | |
| 3.2.1.1 | Co2+ | 5 mM, 16% inhibition | Bacillus subtilis | |
| 3.2.1.1 | Cu2+ | 5 mM CuSO4, 16% inhibition | Bacillus subtilis | |
| 3.2.1.1 | Fe2+ | 5 mM FeSO4, 71% inhibition | Bacillus subtilis | |
| 3.2.1.1 | Hg2+ | 5 mM HgCl2, 98% inhibition | Bacillus subtilis | |
| 3.2.1.1 | Ni2+ | 5 mM NiCl2, 52% inhibition | Bacillus subtilis | |
| 3.2.1.1 | Sr2+ | 5 mM SrCl2, 36% inhibition | Bacillus subtilis | |
| 3.2.1.1 | Zn2+ | 5 mM ZnSO4, 95% inhibition | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 25000 | - |
gel filtration | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | - |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | amylopectin + H2O | - |
Bacillus subtilis | additional information | maltotriose | ? | |
| 3.2.1.1 | amylose + H2O | short chain, DP 17 | Bacillus subtilis | additional information | yield of maltotriose is 69% | ? | |
| 3.2.1.1 | glycogen + H2O | - |
Bacillus subtilis | additional information | maltotriose | ? | |
| 3.2.1.1 | starch + H2O | soluble starch | Bacillus subtilis | additional information | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 50 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 23 | 50 | 23°C: about 50% of maximal activity, 50°C: about 30% of maximal activity | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 45 | - |
unstable above | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6 | 7 | - |
Bacillus subtilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5 | 8 | pH 5.0: about 65% of maximal activity, pH 8.0: about 55% of maximal activity | Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6 | 8 | 25°C, 5 h, stable | Bacillus subtilis |
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