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Literature summary extracted from

  • Saha, B.C.; Bothast, R.J.
    Production and characteristics of an intracellular alpha-glucosidase from a color variant strain of Aureobasidium pullulans (1993), Curr. Microbiol., 27, 73-77.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 1.85
-
 maltose pH 4.0, 50°C Aureobasidium pullulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 intracellular
-
 Aureobasidium pullulans 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Aureobasidium pullulans
-
 color variant strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Aureobasidium pullulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 316.8
-
-
 Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltose + H2O
-
 Aureobasidium pullulans 2 alpha-D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Aureobasidium pullulans maltopentaose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Aureobasidium pullulans alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Aureobasidium pullulans alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Aureobasidium pullulans maltose + D-glucose
-
 ?
3.2.1.20 starch + H2O little raw starch degradation Aureobasidium pullulans alpha-D-glucose
-
 ?
3.2.1.20 sucrose + H2O
-
 Aureobasidium pullulans D-fructose + alpha-D-glucose
-
 ?
3.2.1.20 trehalose + H2O
-
 Aureobasidium pullulans alpha-D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 60
-
-
 Aureobasidium pullulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 45 70 40°C: about 50% of maximal activity, 70°C: about 45% of maximal activity Aureobasidium pullulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 60
-
 1 h, fully stable up to Aureobasidium pullulans
3.2.1.20 65
-
 1 h, 20% loss of activity Aureobasidium pullulans
3.2.1.20 70
-
 1 h, 90% loss of activity Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4
-
-
 Aureobasidium pullulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 3 5 pH 3.0: 90% of maximal activity, pH 5.0: about 65% of maximal activity, no activity at pH 6.5 Aureobasidium pullulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 3 6 50°C, 1 h, completely stable Aureobasidium pullulans