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Literature summary extracted from

  • Kita, A.; Matsui, H.; Somoto, A.; Kimura, A.; Takata, M.; Chiba, S.
    Substrate specificity and subsite affinities of crystalline alpha-glucosidase from Aspergillus niger (1991), Agric. Biol. Chem., 55, 2327-2335.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.20
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.34
-
 phenyl alpha-glucoside
-
 Aspergillus niger
3.2.1.20 0.69
-
 maltotriose
-
 Aspergillus niger
3.2.1.20 0.75
-
 maltose
-
 Aspergillus niger
3.2.1.20 0.87
-
 Phenyl alpha-maltoside
-
 Aspergillus niger
3.2.1.20 1.1
-
 maltotetraose
-
 Aspergillus niger
3.2.1.20 1.9
-
 maltopentaose
-
 Aspergillus niger
3.2.1.20 3.2
-
 maltopentaose
-
 Aspergillus niger
3.2.1.20 4.3
-
 maltodextrin DP = 17 Aspergillus niger
3.2.1.20 4.6
-
 kojibiose
-
 Aspergillus niger
3.2.1.20 4.9
-
 maltohexaose
-
 Aspergillus niger
3.2.1.20 5.3
-
 maltoheptaose
-
 Aspergillus niger
3.2.1.20 8
-
 isomaltose
-
 Aspergillus niger
3.2.1.20 11
-
 maltooctaose
-
 Aspergillus niger
3.2.1.20 12
-
 nigerose
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 125000
-
 x * 125000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.20 glycoprotein contains 25.5% carbohydrate Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 amylose + H2O
-
 Aspergillus niger alpha-D-glucose
-
 ?
3.2.1.20 isomaltose + H2O
-
 Aspergillus niger 2 alpha-D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Aspergillus niger alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltodextrin + H2O DP = 17 Aspergillus niger alpha-D-glucose
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Aspergillus niger maltohexaose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Aspergillus niger maltopentaose + D-glucose
-
 ?
3.2.1.20 maltooctaose + H2O
-
 Aspergillus niger alpha-D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Aspergillus niger alpha-D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Aspergillus niger alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Aspergillus niger alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Aspergillus niger maltose + D-glucose
-
 ?
3.2.1.20 nigerose + H2O
-
 Aspergillus niger 2 alpha-D-glucose
-
 ?
3.2.1.20 phenyl alpha-D-glucoside + H2O
-
 Aspergillus niger phenol + alpha-D-glucose
-
 ?
3.2.1.20 phenyl alpha-glucoside + H2O
-
 Aspergillus niger alpha-D-glucose + phenol
-
 ?
3.2.1.20 phenyl alpha-maltoside + H2O
-
 Aspergillus niger alpha-D-glucose + phenyl alpha-D-glucoside
-
 ?
3.2.1.20 starch + H2O soluble Aspergillus niger alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 ? x * 125000, SDS-PAGE Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 55
-
 15 min, stable up to Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 1.83
-
 phenyl alpha-glucoside
-
 Aspergillus niger
3.2.1.20 32.3
-
 kojibiose
-
 Aspergillus niger
3.2.1.20 50.5
-
 isomaltose
-
 Aspergillus niger
3.2.1.20 88.4
-
 nigerose
-
 Aspergillus niger
3.2.1.20 127
-
 maltooctaose
-
 Aspergillus niger
3.2.1.20 127
-
 maltodextrin DP = 17 Aspergillus niger
3.2.1.20 144
-
 maltose
-
 Aspergillus niger
3.2.1.20 146
-
 maltoheptaose
-
 Aspergillus niger
3.2.1.20 147
-
 maltopentaose
-
 Aspergillus niger
3.2.1.20 163
-
 starch soluble Aspergillus niger
3.2.1.20 170
-
 maltohexaose
-
 Aspergillus niger
3.2.1.20 181
-
 maltotriose
-
 Aspergillus niger
3.2.1.20 181
-
 Phenyl alpha-maltoside
-
 Aspergillus niger
3.2.1.20 193
-
 maltotetraose
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.3
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 3.5 8 4°C, 24 h, stable Aspergillus niger