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Literature summary extracted from

  • Giudicelli, J.; Emiliozzi, R.; Vannier, C.; de Burlet, G.; Sudaka, P.
    Purification by affinity chromatography and characterization of a neutral alpha-glucosidase from horse kidney (1980), Biochim. Biophys. Acta, 612, 85-96.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 carbodiimide
-
 Equus caballus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.1
-
 maltotetraose
-
 Equus caballus
3.2.1.20 0.14
-
 maltopentaose
-
 Equus caballus
3.2.1.20 0.18
-
 maltotriose
-
 Equus caballus
3.2.1.20 0.45
-
 maltose
-
 Equus caballus
3.2.1.20 2.7
-
 p-nitrophenyl alpha-D-glucopyranoside
-
 Equus caballus
3.2.1.20 3.3
-
 nigerose
-
 Equus caballus
3.2.1.20 8.3
-
 kojibiose
-
 Equus caballus
3.2.1.20 13
-
 isomaltose
-
 Equus caballus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 280000
-
 gel filtration Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Equus caballus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.20 glycoprotein
-
 Equus caballus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Equus caballus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 kidney
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 29.3
-
-
 Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltose + H2O
-
 Equus caballus 2 alpha-D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Equus caballus alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Equus caballus alpha-D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Equus caballus alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Equus caballus alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Equus caballus maltose + D-glucose
-
 ?
3.2.1.20 nigerose + H2O
-
 Equus caballus 2 alpha-D-glucose
-
 ?
3.2.1.20 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Equus caballus 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 starch + H2O
-
 Equus caballus alpha-D-glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6
-
 glucoamylase Equus caballus
3.2.1.20 6.25
-
 maltase Equus caballus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 6 9 more than 80% of maximal activity at pH 6 and at pH 9 Equus caballus