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Literature summary extracted from

  • Shima, S.; Goubeaud, M.; Vinzenz, D.; Thauer, R.K.; Ermler, U.
    Crystallization and preliminary X-ray diffraction studies of methyl-coenzyme M reductase from methanobacterium thermoautotrophicum (1997), J. Biochem., 121, 829-830.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.4.1 hanging drop vapor diffusion method, crystal form M obtained with 2-methyl-2,4-pentanediol grown within two months, form P grows from polyethylene glycol 400 within two weeks at 4°C, both crystal forms have one molecule per assymetric unit Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Ni(2+)
-
 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.1 300000
-
-
 Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus final step in methane formation in all methanogenic archaea methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 final step in methane formation in all methanogenic archaea methane + CoM-S-S-CoB
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter thermautotrophicus
-
-
-
2.8.4.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 anaerobic conditions Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B final step in methane formation in all methanogenic archaea Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B final step in methane formation in all methanogenic archaea Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.1 hexamer alpha2,beta2,gamma2 Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 2 mol coenzyme F-430/mol enzyme Methanothermobacter thermautotrophicus
2.8.4.1 F-430 2 mol nickel porphinoid/mol enzyme, prosthetic group Methanothermobacter thermautotrophicus