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Literature summary extracted from

  • Jetten, M.S.M.; Stams, A.J.M.; Zehnder, A.J.B.
    Purification and some properties of the methyl-CoM reductase of Methanothrix soehngenii (1990), FEMS Microbiol. Lett., 66, 183-186.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.4.1 Ti(III) citrate prevents enzyme inactivation by oxygen Methanothrix soehngenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.1 0.023
-
 coenzyme B
-
 Methanothrix soehngenii
2.8.4.1 2
-
 methyl-coenzyme M
-
 Methanothrix soehngenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.1 30400
-
 1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE Methanothrix soehngenii
2.8.4.1 41700
-
 1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE Methanothrix soehngenii
2.8.4.1 63900
-
 1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE Methanothrix soehngenii
2.8.4.1 280000
-
 gel filtration Methanothrix soehngenii

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothrix soehngenii
-
 DSM 2139
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 Q-Sepharose, Phenyl-Superose HR 5/5, Superose 6 HR 10/30 column Methanothrix soehngenii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.4.1 0.051
-
-
 Methanothrix soehngenii

Storage Stability

EC Number Storage Stability Organism
2.8.4.1 -20°C, stored under N2 atmosphere, 50 mM anaerobic Tris-HCl, pH 7.6, 150 mM NaCl, 1 mM Ti(III) citrate Methanothrix soehngenii
2.8.4.1 4°C Methanothrix soehngenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl-coenzyme M + coenzyme B
-
 Methanothrix soehngenii methane + CoM-S-S-CoB
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.1 trimer 1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE Methanothrix soehngenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 37
-
 assay at Methanothrix soehngenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.4.1 7
-
 60 nmol of methane are formed per minute per mg protein Methanothrix soehngenii

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 contains two tightly bound molecules of coenzyme F-430 Methanothrix soehngenii