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Literature summary extracted from

  • Goubeau, M.; Schreiner, G.; Thauer, R.K.
    Purified methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F-430 is reduced to the nickel(I) oxidation state by titanium(III) citrate (1997), Eur. J. Biochem., 243, 110-114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.4.1 Ti(III) citrate conversion from the ox1 to the red1 state, maximal activation after 30 min, 20 mM Ti(III) at pH 9.0 and room temperature or pH 7.0 and 60°C, optimal pH for activation of isoenzyme II is 10.0, enzyme is activated when the enzyme bound coenzyme F-430 is reduced to the Ni(I) state Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Ni(2+)
-
 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.1 300000
-
-
 Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus methanogenesis methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 methanogenesis methane + CoM-S-S-CoB
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter thermautotrophicus
-
-
-
2.8.4.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 4°C, strictly anaerobic conditions, fractional ammonium-sulfate precipitation Methanothermobacter thermautotrophicus
2.8.4.1 copurification of isoenzymes, separation of isoenzymes by anion-exchange chromatography Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.4.1 additional information
-
 activity measured at 92% N2, 8% H2 Methanothermobacter thermautotrophicus
2.8.4.1 100
-
-
 Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
2.8.4.1 4°C, pH 7.0, 4 h, 50% loss of activity of red1 form Methanothermobacter thermautotrophicus
2.8.4.1 storage in frozen stage not possible, thawing leads to a rapid loss of activity Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B methanogenesis Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B methanogenesis Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.1 hexamer alpha2,beta2,gamma2 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 65
-
 assay at Methanothermobacter thermautotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.4.1 60
-
 pH above 9.0 isoenzyme I is slowly denatured Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.4.1 7 7.2 assay at Methanothermobacter thermautotrophicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.4.1 9
-
 above this pH value and 60°C isoenzyme I is slowly denatured Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 2 mol nickel porphinoid/mol enzyme, prosthetic group Methanothermobacter thermautotrophicus