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Literature summary extracted from

  • Hefner, T.; Arend, J.; Warzecha, H.; Siems, K.; Stöckigt, J.
    Arbutin synthase, a novel member of the NRD1beta glycosyltransferase family, is a unique multifunctional enzyme converting various natural products and xenobiotics (2002), Bioorg. Med. Chem., 10, 1731-1741.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.218 cDNA with 6xHis tag Rauvolfia serpentina
2.4.1.218 expression in Escherichia coli Rauvolfia serpentina

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.218 Ca2+ 10 mM, 24.4% inhibition Rauvolfia serpentina
2.4.1.218 Co2+ 10 mM, 54.2% inhibition Rauvolfia serpentina
2.4.1.218 dichlorophen
-
 Rauvolfia serpentina
2.4.1.218 Hg2+ 10 mM, complete inhibition, reversed by addition of 20 mM 2-mercaptoethanol Rauvolfia serpentina
2.4.1.218 Mn2+ 10 mM, 24.7% inhibition Rauvolfia serpentina
2.4.1.218 Zn2+ 10 mM, 87.4% inhibition Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.218 additional information
-
 additional information Km value for hydroquinone is below 0.001 mM Rauvolfia serpentina
2.4.1.218 0.077
-
 UDPglucose
-
 Rauvolfia serpentina
2.4.1.218 0.44
-
 Vanillin
-
 Rauvolfia serpentina
2.4.1.218 0.55
-
 umbelliferone
-
 Rauvolfia serpentina
2.4.1.218 1.1
-
 TDPglucose
-
 Rauvolfia serpentina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.218 Mg2+ 10 mM, increases the conversion rate to 168.3% Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.218 Rauvolfia serpentina Q9AR73
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.218
-
 Rauvolfia serpentina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.218 cell culture
-
 Rauvolfia serpentina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.218 CDP-glucose + hydroquinone 2% of the activity with UDPglucose Rauvolfia serpentina CDP + hydroquinone-O-beta-D-glucopyranoside
-
 ?
2.4.1.218 additional information no activity with UDPmannose, UDPgalactose or UDPglucuronic acid Rauvolfia serpentina ?
-
 ?
2.4.1.218 TDP-glucose + hydroquinone 68.8% of the activity with UDPglucose Rauvolfia serpentina TDP + hydroquinone-O-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-alpha-D-glucose + 3-hydroxyflavone 5.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 3-[(beta-D-glucopyranosyl)oxy]-flavone
-
 ?
2.4.1.218 UDP-alpha-D-glucose + biphenyl-2-ol 1.1% of the activity with hydroquinone Rauvolfia serpentina UDP + [1,1'-biphenyl]-2-yl beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-alpha-D-glucose + kaempferol 1.0% of the activity with hydroquinone Rauvolfia serpentina UDP + ?
-
 ?
2.4.1.218 UDP-alpha-D-glucose + quercetin 2.6% of the activity with hydroquinone Rauvolfia serpentina UDP + ?
-
 ?
2.4.1.218 UDP-glucose + 2-methoxy-hydroquinone 16.7% of the activity with hydroquinone Rauvolfia serpentina UDP + 1-O-(4-hydroxy-2-methoxyphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 3-methoxyphenol 10.9% of the activity with hydroquinone Rauvolfia serpentina UDP + 1-O-(3-methoxyphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-benzyloxyphenol 1.4% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-benzyloxyphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-chloro-2-methylphenol 19.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 1-O-(4-chloro-2-methylphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-chlorophenol 7.5% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-chlorophenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-hydroxyacetophenone ,1.8% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-acetophenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-hydroxybenzaldehyde 8.6% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-formylphenyl beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-hydroxythiophenol 1.2% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-thiophenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-methylphenol 2.8% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-methylphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 4-nitrophenol 6.2% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-nitrophenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + 7-hydroxyflavone 3.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 7-beta-D-glucopyranosyloxy-2-phenylchromen-4-one
-
 ?
2.4.1.218 UDP-glucose + apigenin 5.8% of the activity with hydroquinone Rauvolfia serpentina UDP + ?
-
 ?
2.4.1.218 UDP-glucose + carvacrol 1.0% of the activity with hydroquinone Rauvolfia serpentina UDP + (2-methyl-5-isopropylphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + coniferyl alcohol 1.2% of the activity with hydroquinone Rauvolfia serpentina UDP + coniferin
-
 ?
2.4.1.218 UDP-glucose + eugenol 6.2% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-allyl-2-methoxyphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + hydroquinone
-
 Rauvolfia serpentina UDP + hydroquinone-O-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + methylvanillate 2.2% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid
-
 ?
2.4.1.218 UDP-glucose + phenol 5.1% of the activity with hydroquinone Rauvolfia serpentina UDP + phenyl-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + resorcinol 8.7% of the activity with hydroquinone Rauvolfia serpentina UDP + (3-hydroxyphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + saligenin 9.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 2-(hydroxymethyl)phenyl beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + scopoletin 3.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 7-beta-D-glucopyranosyloxy-6-methoxychromen-2-one
-
 ?
2.4.1.218 UDP-glucose + thymol 7.6% of the activity with hydroquinone Rauvolfia serpentina UDP + (2-isopropyl-5-methyl-phenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + umbelliferone 3.6% of the activity with hydroquinone Rauvolfia serpentina UDP + 7-beta-D-glucopyranosyloxy-chromen-2-one
-
 ?
2.4.1.218 UDP-glucose + vanillic acid 1.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid
-
 ?
2.4.1.218 UDP-glucose + vanillin 9.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-beta-D-glucopyranosyloxy-3-methoxy-benzaldehyde
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.218 50
-
 recombinant enzyme Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.218 4.5
-
 and a second optimum at pH 6.8 in phosphate buffer, and a second optimum at pH 7.5 in citric acid buffer Rauvolfia serpentina
2.4.1.218 6.8
-
 and a second optimum at pH 4.5, phosphate buffer Rauvolfia serpentina
2.4.1.218 7.5
-
 and a second optimum at pH 4.5, citric acid buffer Rauvolfia serpentina