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Literature summary extracted from
- Repair of oxidized proteins: identification of a new methionine sulfoxide reductase (2001), J. Biol. Chem., 276, 48915-48920.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | gene msrA, expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme | Escherichia coli |
| 1.8.4.12 | gene msrB, overexpression of wild-type enzyme and mutant enzymes in Escherichia coli strain DH5alpha | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | enzyme is involved in repairing of oxidized methionine residues in proteins | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| 1.8.4.11 | additional information | Escherichia coli | physiological role | ? | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Escherichia coli | enzyme is involved in repairing of oxidized methionine residues in proteins | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | Escherichia coli | enzyme contributes to resistance against cadmium, physiological role | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Escherichia coli | - |
gene msrA, enzyme is organized in a fusion protein together with MsrB, EC 1.8.4.B1 | - |
| 1.8.4.12 | Escherichia coli | - |
gene msrB, enzyme SelR which is a selenoprotein, enzyme is organized in a fusion protein together with MsrA, EC 1.8.4.B2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Escherichia coli |
| 1.8.4.12 | recombinant enzyme from Escherichia coli strain DH5alpha | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 0.33 | - |
in vitro, substrate free L-methionine (R)-sulfoxide | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | enzyme is involved in repairing of oxidized methionine residues in proteins | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-form of the substrate | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | physiological role | Escherichia coli | ? | - |
? | |
| 1.8.4.11 | oxidized calmodulin + thioredoxin | enzyme reduces L-methionine (S)-sulfoxide of the protein substrate | Escherichia coli | partially reduced calmodulin + thioredoxin disulfide | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | enzyme is involved in repairing of oxidized methionine residues in proteins | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form of the substrate | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | enzyme contributes to resistance against cadmium, physiological role | Escherichia coli | ? | - |
? | |
| 1.8.4.12 | oxidized calmodulin + thioredoxin | enzyme reduces L-methionine (R)-sulfoxide of the protein substrate | Escherichia coli | partially reduced calmodulin + thioredoxin disulfide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.4.12 | More | MsrB has a highly conserved sequence among organisms | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | methionine sulfoxide reductase | - |
Escherichia coli |
| 1.8.4.11 | MsrA | - |
Escherichia coli |
| 1.8.4.12 | methionine sulfoxide reductase | - |
Escherichia coli |
| 1.8.4.12 | MsrB | - |
Escherichia coli |
| 1.8.4.12 | SelR | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.12 | 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.12 | 0.01 | - |
L-methionine (R)-sulfoxide | in vitro, substrate free L-methionine (R)-sulfoxide | Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.12 | 7.5 | - |
assay at | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | thioredoxin | - |
Escherichia coli | |
| 1.8.4.12 | thioredoxin | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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