BRENDA - Enzyme Database show

A specific enzymatic reduction of L(-)methionine sulfoxide and a related nonspecific reduction of disulfides

Black, S.; Harte, E.M.; Hudson, B.; Wartolofsky, L.; J. Biol. Chem. 235, 2910-2916 (1960)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.4.13
4-chloromercuribenzoate
complete inhibition at 1 mM
Saccharomyces cerevisiae
1.8.4.13
arsenite
strong inhibition
Saccharomyces cerevisiae
1.8.4.13
bovine serum albumin
-
Saccharomyces cerevisiae
1.8.4.13
DL-methionine sulfoximine
50% inhibition at 1 mM
Saccharomyces cerevisiae
1.8.4.13
hydroxyethyl disulfide
competitively inhibits the activity with L-(-)-methionine S-oxide about 2.5fold
Saccharomyces cerevisiae
1.8.4.13
iodoacetic acid
complete inhibition at 10 mM
Saccharomyces cerevisiae
1.8.4.13
L-(-)-methionine S-oxide
competitively inhibits the activity with a disulfide about 2.5fold
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.4.13
0.2
-
L-(-)-methionine S-oxide
pH 7.0, 22°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.4.13
L-(-)-methionine S-oxide + NADPH
Saccharomyces cerevisiae
the enzyme is specific for the L-(-)-stereoisomer
L-methionine + NADP+ + H2O
-
-
ir
1.8.4.13
additional information
Saccharomyces cerevisiae
possible pathway, overview
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.4.13
Saccharomyces cerevisiae
-
two enzyme forms
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.4.13
two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.4.13
L-(-)-methionine S-oxide + NADPH
the enzyme is specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
1.8.4.13
L-(-)-methionine S-oxide + NADPH
the enzyme is absolutely specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
1.8.4.13
additional information
possible pathway, overview
393070
Saccharomyces cerevisiae
?
-
-
-
-
1.8.4.13
additional information
the purified enzyme may contain thioredoxin
393070
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.13
22
-
assay at room temperature
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.13
7
-
-
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.4.13
NADPH
specific for
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.4.13
NADPH
specific for
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.4.13
4-chloromercuribenzoate
complete inhibition at 1 mM
Saccharomyces cerevisiae
1.8.4.13
arsenite
strong inhibition
Saccharomyces cerevisiae
1.8.4.13
bovine serum albumin
-
Saccharomyces cerevisiae
1.8.4.13
DL-methionine sulfoximine
50% inhibition at 1 mM
Saccharomyces cerevisiae
1.8.4.13
hydroxyethyl disulfide
competitively inhibits the activity with L-(-)-methionine S-oxide about 2.5fold
Saccharomyces cerevisiae
1.8.4.13
iodoacetic acid
complete inhibition at 10 mM
Saccharomyces cerevisiae
1.8.4.13
L-(-)-methionine S-oxide
competitively inhibits the activity with a disulfide about 2.5fold
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.4.13
0.2
-
L-(-)-methionine S-oxide
pH 7.0, 22°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.4.13
L-(-)-methionine S-oxide + NADPH
Saccharomyces cerevisiae
the enzyme is specific for the L-(-)-stereoisomer
L-methionine + NADP+ + H2O
-
-
ir
1.8.4.13
additional information
Saccharomyces cerevisiae
possible pathway, overview
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.4.13
two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.4.13
L-(-)-methionine S-oxide + NADPH
the enzyme is specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
1.8.4.13
L-(-)-methionine S-oxide + NADPH
the enzyme is absolutely specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
1.8.4.13
additional information
possible pathway, overview
393070
Saccharomyces cerevisiae
?
-
-
-
-
1.8.4.13
additional information
the purified enzyme may contain thioredoxin
393070
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.13
22
-
assay at room temperature
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.13
7
-
-
Saccharomyces cerevisiae