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Literature summary extracted from

  • Black, S.; Harte, E.M.; Hudson, B.; Wartolofsky, L.
    A specific enzymatic reduction of L(-)methionine sulfoxide and a related nonspecific reduction of disulfides (1960), J. Biol. Chem., 235, 2910-2916.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.4.13 4-chloromercuribenzoate complete inhibition at 1 mM Saccharomyces cerevisiae
1.8.4.13 arsenite strong inhibition Saccharomyces cerevisiae
1.8.4.13 bovine serum albumin
-
 Saccharomyces cerevisiae
1.8.4.13 DL-methionine sulfoximine 50% inhibition at 1 mM Saccharomyces cerevisiae
1.8.4.13 hydroxyethyl disulfide competitively inhibits the activity with L-(-)-methionine S-oxide about 2.5fold Saccharomyces cerevisiae
1.8.4.13 iodoacetic acid complete inhibition at 10 mM Saccharomyces cerevisiae
1.8.4.13 L-(-)-methionine S-oxide competitively inhibits the activity with a disulfide about 2.5fold Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.13 0.2
-
 L-(-)-methionine S-oxide pH 7.0, 22°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.13 L-(-)-methionine S-oxide + NADPH Saccharomyces cerevisiae the enzyme is specific for the L-(-)-stereoisomer L-methionine + NADP+ + H2O
-
 ir
1.8.4.13 additional information Saccharomyces cerevisiae possible pathway, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.13 Saccharomyces cerevisiae
-
 two enzyme forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.13 two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.13 L-(-)-methionine S-oxide + NADPH the enzyme is specific for the L-(-)-stereoisomer Saccharomyces cerevisiae L-methionine + NADP+ + H2O
-
 ir
1.8.4.13 L-(-)-methionine S-oxide + NADPH the enzyme is absolutely specific for the L-(-)-stereoisomer Saccharomyces cerevisiae L-methionine + NADP+ + H2O
-
 ir
1.8.4.13 additional information possible pathway, overview Saccharomyces cerevisiae ?
-
 ?
1.8.4.13 additional information the purified enzyme may contain thioredoxin Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.13 22
-
 assay at room temperature Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.13 7
-
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.13 NADPH specific for Saccharomyces cerevisiae