BRENDA - Enzyme Database show

Hepatic sulfite oxidase. Purification and properties

Cohen, H.J.; Fridovich, I.; J. Biol. Chem. 246, 359-366 (1971)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.3.1
2,6-dichloroindophenol
inhibition of O2 consumption
Bos taurus
1.8.3.1
arsenite
-
Bos taurus
1.8.3.1
CN-
-
Bos taurus
1.8.3.1
cytochrome c
inhibition of O2 consumption
Bos taurus
1.8.3.1
ferricyanide
inhibition of O2 consumption
Bos taurus
1.8.3.1
mannitol
only with O2 as electron acceptor
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.3.1
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.3.1
75% pure
Bos taurus
Reaction
EC Number
Reaction
Commentary
Organism
1.8.3.1
sulfite + O2 + H2O = sulfate + H2O2
ping-pong mechanism
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.3.1
sulfite + H2O + A
A: electron acceptor, i.e. O2, cytochrome c, K3[Fe(CN)6], 2,6-dichloroindophenol, methylene blue, highly specific for sulfite as electron donor
393014
Bos taurus
SO42- + AH2
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.8.3.1
52
-
inactivation, protection by sulfate
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.3.1
8.6
-
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.3.1
2,6-dichloroindophenol
inhibition of O2 consumption
Bos taurus
1.8.3.1
arsenite
-
Bos taurus
1.8.3.1
CN-
-
Bos taurus
1.8.3.1
cytochrome c
inhibition of O2 consumption
Bos taurus
1.8.3.1
ferricyanide
inhibition of O2 consumption
Bos taurus
1.8.3.1
mannitol
only with O2 as electron acceptor
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.3.1
75% pure
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.3.1
sulfite + H2O + A
A: electron acceptor, i.e. O2, cytochrome c, K3[Fe(CN)6], 2,6-dichloroindophenol, methylene blue, highly specific for sulfite as electron donor
393014
Bos taurus
SO42- + AH2
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.8.3.1
52
-
inactivation, protection by sulfate
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.3.1
8.6
-
-
Bos taurus