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Literature summary extracted from

  • Yoshimoto, A.; Naiki, N.; Sato, R.
    Sulfite reductase (bakers¦ yeast) (1971), Methods Enzymol., 17B, 520-528.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.8.1.2 low ionic strength such as 0.01 M phosphate buffer destroys NADPH-dependent activities Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.2 arsenite
-
 Saccharomyces cerevisiae
1.8.1.2 CN- severe inhibition of NADPH-sulfite,-nitrite,-hydroxylamine reductase, no inhibition of methyl viologen-sulfite reductase, small extent of inhibition of NADPH dependent reduction of cytochrome c, ferricyanide, quinones etc. Saccharomyces cerevisiae
1.8.1.2 NADP+ NADPH-dependent activities Saccharomyces cerevisiae
1.8.1.2 p-chloromercuribenzoate NADPH-dependent activities Saccharomyces cerevisiae
1.8.1.2 Sulfide reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.2 0.012 0.017 sulfite
-
 Saccharomyces cerevisiae
1.8.1.2 0.018
-
 NADPH reduction of cytochrome c Saccharomyces cerevisiae
1.8.1.2 0.021
-
 NADPH reduction of sulfite Saccharomyces cerevisiae
1.8.1.2 0.06
-
 cytochrome c
-
 Saccharomyces cerevisiae
1.8.1.2 0.8 1.5 nitrite
-
 Saccharomyces cerevisiae
1.8.1.2 4.5 10.5 hydroxylamine
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.2 soluble
-
 Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.2 350000
-
 calculation from FAD and FMN content, sedimentation coefficient Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.2 sulfite + NADPH + H+ Saccharomyces cerevisiae
-
 sulfide + NADP+ + H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.8.1.2 Acetone inactivation Saccharomyces cerevisiae
1.8.1.2 Ethanol inactivation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.2 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.2
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.8.1.2 -20°C, 0.3 M phosphate buffer pH 7.3, 1 year stable stable Saccharomyces cerevisiae
1.8.1.2 4°C, 0.3 M phosphate buffer pH 7.3, 1 week stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.2 hydroxylamine + NADPH
-
 Saccharomyces cerevisiae ammonia + NADP+
-
 ?
1.8.1.2 additional information overview on assay methods Saccharomyces cerevisiae ?
-
 ?
1.8.1.2 additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Saccharomyces cerevisiae ?
-
 ?
1.8.1.2 NADP+ + ferrocytochrome c
-
 Saccharomyces cerevisiae NADPH + H+ + ferricytochrome c
-
 r
1.8.1.2 nitrite + NADPH
-
 Saccharomyces cerevisiae ammonia + NADP+
-
 ?
1.8.1.2 sulfite + NADPH
-
 Saccharomyces cerevisiae sulfide + NADP+ + H2O
-
 ?
1.8.1.2 sulfite + NADPH + H+
-
 Saccharomyces cerevisiae sulfide + NADP+ + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.2 7 7.5 NADPH-sulfite reductase Saccharomyces cerevisiae
1.8.1.2 7.5 7.9 NADPH-cytochrome c reductase Saccharomyces cerevisiae
1.8.1.2 7.7 8.5 methyl viologen-sulfite reductase Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.2 flavin treatment with ammonium sulfate causes dissociation of FMN but retetion of FAD and heme Saccharomyces cerevisiae