BRENDA - Enzyme Database show

TPNH-Sulfite reductase (Escherichia coli)

Siegel, L.M.; Kamin, H.; Methods Enzymol. 17B, 539-545 (1971)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.2
arsenite
-
Escherichia coli
1.8.1.2
CN-
-
Escherichia coli
1.8.1.2
CO
-
Escherichia coli
1.8.1.2
NADP+
NADPH-dependent activities
Escherichia coli
1.8.1.2
p-Mercuriphenylsulfonate
NADPH-dependent reactions
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.2
0.0043
0.0074
sulfite
-
Escherichia coli
1.8.1.2
0.018
-
NADPH
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.2
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.2
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.2
2.73
2.87
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.8.1.2
-15°C, 0.05 M potassium phosphate buffer pH 7.7, 10 mM EDTA, at least 6 months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.2
hydroxylamine + NADPH
-
392968
Escherichia coli
ammonia + NADP+
-
392968
Escherichia coli
?
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
392968
Escherichia coli
?
-
-
-
-
1.8.1.2
nitrite + NADPH
-
392968
Escherichia coli
ammonia + NADP+
-
392968
Escherichia coli
?
1.8.1.2
sulfite + NADPH
-
392968
Escherichia coli
sulfide + NADP+ + H2O
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.1.2
30
40
sulfite
cosubstrate NADPH
Escherichia coli
1.8.1.2
43.3
51.7
nitrite
cosubstrate NADPH
Escherichia coli
1.8.1.2
98.3
217
hydroxylamine
cosubstrate NADPH
Escherichia coli
1.8.1.2
150
-
FAD
cosubstrate NADPH
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.2
7.2
-
electron donor NADPH
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.1.2
0.006
0.009
cyanide
-
Escherichia coli
1.8.1.2
0.08
0.12
arsenite
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.2
arsenite
-
Escherichia coli
1.8.1.2
CN-
-
Escherichia coli
1.8.1.2
CO
-
Escherichia coli
1.8.1.2
NADP+
NADPH-dependent activities
Escherichia coli
1.8.1.2
p-Mercuriphenylsulfonate
NADPH-dependent reactions
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.1.2
0.006
0.009
cyanide
-
Escherichia coli
1.8.1.2
0.08
0.12
arsenite
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.2
0.0043
0.0074
sulfite
-
Escherichia coli
1.8.1.2
0.018
-
NADPH
-
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.2
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.2
2.73
2.87
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.1.2
-15°C, 0.05 M potassium phosphate buffer pH 7.7, 10 mM EDTA, at least 6 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.2
hydroxylamine + NADPH
-
392968
Escherichia coli
ammonia + NADP+
-
392968
Escherichia coli
?
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
392968
Escherichia coli
?
-
-
-
-
1.8.1.2
nitrite + NADPH
-
392968
Escherichia coli
ammonia + NADP+
-
392968
Escherichia coli
?
1.8.1.2
sulfite + NADPH
-
392968
Escherichia coli
sulfide + NADP+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.1.2
30
40
sulfite
cosubstrate NADPH
Escherichia coli
1.8.1.2
43.3
51.7
nitrite
cosubstrate NADPH
Escherichia coli
1.8.1.2
98.3
217
hydroxylamine
cosubstrate NADPH
Escherichia coli
1.8.1.2
150
-
FAD
cosubstrate NADPH
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.2
7.2
-
electron donor NADPH
Escherichia coli