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Literature summary extracted from

  • Sengupta, S.; Shaila, M.S.; Rao, G.R.
    In vitro and in vivo regulation of assimilatory nitrite reductase from Candida utilis (1997), Arch. Microbiol., 168, 215-224.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.4 iodoacetate 64% inhibition Cyberlindnera jadinii
1.7.1.4 N-ethylmaleimide 1 mM, 64% inhibition after 5 min, reduced glutathione restores activity to 94% Cyberlindnera jadinii
1.7.1.4 p-chloromercuribenzoate 1 mM, 79% inhibition after 5 min, dithiothreitol completely reverses activity, reduced glutathione, L-cysteine and 2-mercaptoethanol reverse activity to 86%, 64% and 82% respectively Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Cyberlindnera jadinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.4
-
 Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.4 1800
-
 activity in crude extracts of cells grown in the presence of 50 mM urea Cyberlindnera jadinii
1.7.1.4 5900
-
 activity in crude extracts of cells grown in the presence of 50 mM glutamine Cyberlindnera jadinii
1.7.1.4 17300
-
 activity in crude extracts of cells grown in the presence of 50 mM ammonium sulfate Cyberlindnera jadinii
1.7.1.4 24000
-
 activity in crude extracts of cells grown in the presence of 50 mM asparagine Cyberlindnera jadinii
1.7.1.4 60000
-
 activity in crude extracts of cells grown in the presence of 50 mM potassium nitrate Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+ enzyme can utilize NAD(P)H and methyl viologen as electron donors, NADPH is more effective than NADH Cyberlindnera jadinii NAD(P)+ + NH3 + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 FAD addition increases activity Cyberlindnera jadinii
1.7.1.4 FMN 0.01 mM, approx. 2fold increase in activity Cyberlindnera jadinii
1.7.1.4 NADH
-
 Cyberlindnera jadinii
1.7.1.4 NADPH
-
 Cyberlindnera jadinii