Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lafferty, M.A.; Garrett, R.H.
    Purification and properties of the Neurospora crassa assimilatory nitrite reductase (1974), J. Biol. Chem., 249, 7555-7567.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.4 1,10-phenanthroline
-
 Neurospora crassa
1.7.1.4 CN-
-
 Neurospora crassa
1.7.1.4 hydroxylamine
-
 Neurospora crassa
1.7.1.4 KCN
-
 Neurospora crassa
1.7.1.4 Na2S
-
 Neurospora crassa
1.7.1.4 NAD(P)H
-
 Neurospora crassa
1.7.1.4 p-hydroxymercuribenzoate very potent inhibitor, inhibition can be reversed by either cysteine or glutathione Neurospora crassa
1.7.1.4 sodium disulfite
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.4 0.0001
-
 FAD
-
 Neurospora crassa
1.7.1.4 0.01
-
 NO2- cosubstrate NADPH or NADH Neurospora crassa
1.7.1.4 0.8
-
 NO2- + dithionite Neurospora crassa
1.7.1.4 2.5
-
 hydroxylamine
-
 Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.4 290000
-
 gel filtration, sucrose density gradient centrifugation Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+ Neurospora crassa
-
 NAD(P)+ + NH3 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.4
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.4 0.52
-
 NADH + NO2- Neurospora crassa
1.7.1.4 0.73
-
 NADPH + NO2- Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + NH2OH
-
 Neurospora crassa NAD(P)+ + NH3 + H2O
-
 ?
1.7.1.4 NAD(P)H + nitrite + H+
-
 Neurospora crassa NAD(P)+ + NH3 + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.4 40
-
 80% activity is lost after 4 min Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.4 7.5
-
-
 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 NADH
-
 Neurospora crassa
1.7.1.4 NADPH
-
 Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.4 0.002
-
 p-hydroxymercuribenzoate
-
 Neurospora crassa
1.7.1.4 0.005
-
 KCN
-
 Neurospora crassa
1.7.1.4 2
-
 1,10-phenanthroline
-
 Neurospora crassa
1.7.1.4 2
-
 sodium disulfite
-
 Neurospora crassa
1.7.1.4 5
-
 Sodium sulfide
-
 Neurospora crassa
1.7.1.4 6
-
 hydroxylamine
-
 Neurospora crassa