BRENDA - Enzyme Database show

Preparation and some properties of homogeneous Neurospora crassa assimilatory NADPH-nitrite reductase

Greenbaum, P.; Prodouz, K.N.; Garrett, R.H.; Biochim. Biophys. Acta 526, 52-64 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.4
8-hydroxyquinoline
-
Neurospora crassa
1.7.1.4
arsenite
-
Neurospora crassa
1.7.1.4
CN-
-
Neurospora crassa
1.7.1.4
CO
almost complete inhibition
Neurospora crassa
1.7.1.4
p-chloromercuribenzoate
-
Neurospora crassa
1.7.1.4
SO32-
-
Neurospora crassa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.7.1.4
Neurospora crassa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.7.1.4
-
Neurospora crassa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.7.1.4
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.4
26.9
-
-
Neurospora crassa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.4
NAD(P)H + NH2OH
NADPH is preferred
392931
Neurospora crassa
NAD(P)+ + NH3 + H2O
-
392931
Neurospora crassa
?
1.7.1.4
NAD(P)H + nitrite + H+
NADPH is preferred
392931
Neurospora crassa
NAD(P)+ + NH3 + H2O
-
392931
Neurospora crassa
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7.1.4
130
-
NO2-
-
Neurospora crassa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.4
NADH
-
Neurospora crassa
1.7.1.4
NADPH
-
Neurospora crassa
1.7.1.4
siroheme
-
Neurospora crassa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.4
NADH
-
Neurospora crassa
1.7.1.4
NADPH
-
Neurospora crassa
1.7.1.4
siroheme
-
Neurospora crassa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.4
8-hydroxyquinoline
-
Neurospora crassa
1.7.1.4
arsenite
-
Neurospora crassa
1.7.1.4
CN-
-
Neurospora crassa
1.7.1.4
CO
almost complete inhibition
Neurospora crassa
1.7.1.4
p-chloromercuribenzoate
-
Neurospora crassa
1.7.1.4
SO32-
-
Neurospora crassa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.1.4
-
Neurospora crassa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.7.1.4
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.4
26.9
-
-
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.4
NAD(P)H + NH2OH
NADPH is preferred
392931
Neurospora crassa
NAD(P)+ + NH3 + H2O
-
392931
Neurospora crassa
?
1.7.1.4
NAD(P)H + nitrite + H+
NADPH is preferred
392931
Neurospora crassa
NAD(P)+ + NH3 + H2O
-
392931
Neurospora crassa
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7.1.4
130
-
NO2-
-
Neurospora crassa