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Literature summary extracted from

  • Lazzarini, R.A.; Atkinson, D.E.
    A triphosphopyridine nucleotide-specific nitrite reductase from Escherichia coli (1961), J. Biol. Chem., 236, 3330-3335.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.1.4 dialysis for 15 h against 1 mM cyanide, 1 mM 8-hydroxyquinoline or 1 mM EDTA followed by dialysis against 0.05 M phosphate buffer, no decrease in activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.4 arsenite 0.5 mM, 62% inhibition Escherichia coli
1.7.1.4 CN- 0.02 mM, 88% inhibition Escherichia coli
1.7.1.4 NADP+ 0.06 mM, 13% inhibition, 0.27 mM, 43% inhibition Escherichia coli
1.7.1.4 p-chloromercuribenzoate 0.001 mM, 83% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.4 0.4
-
 NO2-
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.4 cell associated
-
 Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Escherichia coli
-
-
-
1.7.1.4 Escherichia coli Bn
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.4
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.4 0.18
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+ specific for NADH Escherichia coli NAD(P)+ + NH3 + H2O
-
 ?
1.7.1.4 NAD(P)H + nitrite + H+ specific for NADH Escherichia coli Bn NAD(P)+ + NH3 + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.4 7.5 8.5
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.4 6.5 9.5 pH 6.5: about 50% activity, pH 9.5: about 15% activity Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 NADH enzyme is specific for NADPH, NADH reaction rate approx. 1% of NADPH rate Escherichia coli
1.7.1.4 NADPH enzyme is specific for NADPH, NADH reaction rate approx. 1% of NADPH rate Escherichia coli