BRENDA - Enzyme Database show

Purification and properties of nitrite reductase from Escherichia coli K12

Coleman, K.J.; Cornish-Bowden, A.; Cole, J.A.; Biochem. J. 175, 483-493 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.4
1,10-phenanthroline
-
Escherichia coli
1.7.1.4
2,2'-bipyridine
-
Escherichia coli
1.7.1.4
arsenite
10 mM, 24% inhibition
Escherichia coli
1.7.1.4
CN-
-
Escherichia coli
1.7.1.4
NaN3
-
Escherichia coli
1.7.1.4
p-chloromercuribenzoate
0.02 mM, complete inactivation, partially prevented by 10 mM cysteine
Escherichia coli
1.7.1.4
SO32-
1 mM, 22% inhibition
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.7.1.4
cell associated
-
Escherichia coli
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.4
88000
-
2 * 88000, SDS-PAGE
Escherichia coli
1.7.1.4
190000
-
glycerol density gradient centrifugation
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.7.1.4
NAD(P)H + nitrite + H+
Escherichia coli
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli
?
1.7.1.4
NAD(P)H + nitrite + H+
Escherichia coli OR75Ch15
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli OR75Ch15
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.7.1.4
Escherichia coli
-
-
-
1.7.1.4
Escherichia coli OR75Ch15
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.7.1.4
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.4
16.7
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.4
NAD(P)H + NH2OH
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
1.7.1.4
NAD(P)H + NH2OH
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
1.7.1.4
NAD(P)H + nitrite + H+
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
1.7.1.4
NAD(P)H + nitrite + H+
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
Subunits
EC Number
Subunits
Commentary
Organism
1.7.1.4
dimer
2 * 88000, SDS-PAGE
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.4
flavin
0.4 mol of flavin per mol of enzyme
Escherichia coli
1.7.1.4
additional information
heme is not detectable
Escherichia coli
1.7.1.4
NADH
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.4
flavin
0.4 mol of flavin per mol of enzyme
Escherichia coli
1.7.1.4
additional information
heme is not detectable
Escherichia coli
1.7.1.4
NADH
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.4
1,10-phenanthroline
-
Escherichia coli
1.7.1.4
2,2'-bipyridine
-
Escherichia coli
1.7.1.4
arsenite
10 mM, 24% inhibition
Escherichia coli
1.7.1.4
CN-
-
Escherichia coli
1.7.1.4
NaN3
-
Escherichia coli
1.7.1.4
p-chloromercuribenzoate
0.02 mM, complete inactivation, partially prevented by 10 mM cysteine
Escherichia coli
1.7.1.4
SO32-
1 mM, 22% inhibition
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.7.1.4
cell associated
-
Escherichia coli
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.4
88000
-
2 * 88000, SDS-PAGE
Escherichia coli
1.7.1.4
190000
-
glycerol density gradient centrifugation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.7.1.4
NAD(P)H + nitrite + H+
Escherichia coli
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli
?
1.7.1.4
NAD(P)H + nitrite + H+
Escherichia coli OR75Ch15
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli OR75Ch15
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.1.4
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.4
16.7
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.4
NAD(P)H + NH2OH
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
1.7.1.4
NAD(P)H + NH2OH
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
1.7.1.4
NAD(P)H + nitrite + H+
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
1.7.1.4
NAD(P)H + nitrite + H+
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.7.1.4
dimer
2 * 88000, SDS-PAGE
Escherichia coli