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Literature summary extracted from

  • Nakamura, Y.; Ikawa, T.
    Purification and properties of NADH: nitrate reductase from the red alga Porphyra yezoensis (1993), Plant Cell Physiol., 34, 1239-1249.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 azide inhibition of full and nitrate-reducing partial activities Neopyropia yezoensis
1.7.1.1 cyanide inhibition of full and nitrate-reducing partial activities Neopyropia yezoensis
1.7.1.1 NEM inhibition of full and NADH-utilizing partial activities Neopyropia yezoensis
1.7.1.1 p-hydroxymercuribenzoate inhibition of full and NADH-utilizing partial activities Neopyropia yezoensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.023
-
 NADH
-
 Neopyropia yezoensis
1.7.1.1 0.064
-
 nitrate
-
 Neopyropia yezoensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.1 100000
-
 2 * 100000, SDS-PAGE Neopyropia yezoensis
1.7.1.1 220000
-
 gel filtration Neopyropia yezoensis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Neopyropia yezoensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Neopyropia yezoensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 12.5
-
-
 Neopyropia yezoensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 2 ferricytochrome c + NADH
-
 Neopyropia yezoensis 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.7.1.1 FMNH2 + nitrate
-
 Neopyropia yezoensis FMN + nitrite
-
 ?
1.7.1.1 nitrate + NADH
-
 Neopyropia yezoensis nitrite + NAD+ + H2O
-
 ?
1.7.1.1 nitrate + NADPH no activity Neopyropia yezoensis nitrite + NADP+ + H2O
-
 ?
1.7.1.1 reduced methyl viologen + nitrate
-
 Neopyropia yezoensis methyl viologen + nitrite
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.1 dimer 2 * 100000, SDS-PAGE Neopyropia yezoensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 7
-
 FMNH2-nitrate reductase activity Neopyropia yezoensis
1.7.1.1 7.5
-
 reduced methyl viologen-nitrate reductase activity Neopyropia yezoensis
1.7.1.1 8.3
-
-
 Neopyropia yezoensis
1.7.1.1 8.5
-
 NADH-ferricyanide reductase activity and NADH-cytochrome c reductase activity Neopyropia yezoensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Neopyropia yezoensis