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Literature summary extracted from

  • Oji, Y.; Mamano, T.; Ryoma, Y.; Miki, Y.; Okamoto, S.
    Purification and characterization of NADH-nitrate reductase from leaves of 2-row barley, and its activity as affected by some metabolites (1985), J. Plant Physiol., 119, 247-256.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.1 HPO42- activates Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 ADP competitive with ADP Hordeum vulgare
1.7.1.1 Carbamoyl phosphate competitive Hordeum vulgare
1.7.1.1 Co2+ 1 mM, strong inhibition Hordeum vulgare
1.7.1.1 Cu2+ 1 mM, strong inhibition Hordeum vulgare
1.7.1.1 Mn2+ 1 mM, 27% inhibition Hordeum vulgare
1.7.1.1 MoO42- 1 mM, strong inhibition Hordeum vulgare
1.7.1.1 NO2- 10 mM, 65% inhibition Hordeum vulgare
1.7.1.1 pyridoxal 5'-phosphate uncompetitive with nitrate Hordeum vulgare
1.7.1.1 VO3- 1 mM, strong inhibition Hordeum vulgare
1.7.1.1 Zn2+ 1 mM, strong inhibition Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.0038
-
 NADH
-
 Hordeum vulgare
1.7.1.1 0.27
-
 nitrate
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.1 225000
-
 disc gel electrophoresis Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.1 leaf
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 37
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 2 ferricyanide + NADH
-
 Hordeum vulgare 2 ferrocyanide + NAD+ + H+
-
 ?
1.7.1.1 2 ferricytochrome c + NADH
-
 Hordeum vulgare 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.7.1.1 FADH2 + nitrate
-
 Hordeum vulgare FAD + nitrite
-
 ?
1.7.1.1 FMNH2 + nitrate
-
 Hordeum vulgare FMN + nitrite
-
 ?
1.7.1.1 nitrate + NADH
-
 Hordeum vulgare nitrite + NAD+ + H2O
-
 ?
1.7.1.1 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Hordeum vulgare ? + NAD+
-
 ?
1.7.1.1 reduced methyl viologen + nitrate
-
 Hordeum vulgare methyl viologen + nitrite
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.1
-
-
 pH 7.5, half-life: 42 h Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 7.5
-
-
 Hordeum vulgare

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.1 7.5
-
 0°C, half-life: 42 h Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 FAD required for maximal activity, Km: 8 nM Hordeum vulgare
1.7.1.1 NADH
-
 Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.1 0.018
-
 Carbamoyl phosphate
-
 Hordeum vulgare
1.7.1.1 0.176
-
 pyridoxal 5'-phosphate
-
 Hordeum vulgare
1.7.1.1 1.36
-
 ADP
-
 Hordeum vulgare