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Literature summary extracted from

  • Jagendorf, A.T.
    Chloroplast TPNH diaphorase (1963), Methods Enzymol., 6, 430-434.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.10 2'-AMP
-
 Spinacia oleracea
1.6.5.10 Co2+ 0.5 mM Spinacia oleracea
1.6.5.10 HgCl2 0.02 mM Spinacia oleracea
1.6.5.10 iodoacetate 3 mM Spinacia oleracea
1.6.5.10 PCMB 0.1 mM Spinacia oleracea
1.6.5.10 Zn2+ 0.5 mM Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.10 0.006
-
 NADPH
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.10 chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.10 30000 40000 ultracentrifugal analysis Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.10 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.10 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.10 28.1
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.10 NADH + H+ + acceptor no activity Spinacia oleracea NAD+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor acceptor: ferricyanide Spinacia oleracea NADP+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor acceptor: menadione Spinacia oleracea NADP+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor acceptor: benzoquinone Spinacia oleracea NADP+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor acceptor: ferric chloride Spinacia oleracea NADP+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor acceptor: methylene blue Spinacia oleracea NADP+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor acceptor: FMN Spinacia oleracea NADP+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor aceptor: 1,10-phenanthroline Spinacia oleracea NADP+ + reduced acceptor
-
 ?
1.6.5.10 NADPH + acceptor 2,3',6-trichlorophenolindophenol is reduced to leuco-2,3',6-trichlorophenolindophenol Spinacia oleracea NADP+ + reduced acceptor
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.10 FAD one FAD per enzyme molecule of 35000 Da Spinacia oleracea
1.6.5.10 NADPH
-
 Spinacia oleracea