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Literature summary extracted from

  • Kwan, H.S.; Barrett, E.L.
    Purification and properties of trimethylamine oxide reductase from Salmonella typhimurium (1983), J. Bacteriol., 155, 1455-1458.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.3 Co2+ not inhibitory at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 Cu2+ 50% inhibition at 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 EDTA not inhibitory at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 FAD inhibition, when added to the standard assay mixture containing benzyl viologen as electron acceptor Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 FMN inhibition, when added to the standard assay mixture containing benzyl viologen as electron acceptor Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 Hg2+ 80% inhibition at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 KCN not inhibitory at 5 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 Mn2+ not inhibitory at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 NaN3 not inhibitory at 10 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.3 0.89
-
 Trimethylamine N-oxide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 2.2
-
 Chlorate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.3 84000
-
 4 * 84000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 332000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
 inducible enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.3 using polyethyleneimine to remove nucleic acids, ammonium sulfate treatment, column chromatography on DEAE-Sephacel and Bio-Gel A-5m, chromatofocusing and chromatography on Sephacryl-S-300 column Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.3 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
1.7.2.3 -80°C, 10% loss of activity after 15 days Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 0°C, 50 mM sodium phosphate buffer, pH 7.3, 30% loss of activity after 17 days Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.3 chlorate + electron donor
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
1.7.2.3 additional information when FMN, FAD, cytochrome c, NADPH, NADH or ascorbate are substituted at equimolar concentration for benzyl viologen, none facilitates the oxidation of dithionite by the trimethylamine N-oxide Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
1.7.2.3 additional information the enzyme has very slight activity with nitrate, but no activity with nitrite, sulfite or thiosulfate Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
1.7.2.3 trimethylamine N-oxide + electron donor benzyl viologen as electron donor Salmonella enterica subsp. enterica serovar Typhimurium trimethylamine + oxidized electron donor + H2O
-
 ?
1.7.2.3 trimethylamine N-oxide + electron donor the activity with 3.4 mM FMN is 17% of that obtained with 0.33 mM benzyl viologen Salmonella enterica subsp. enterica serovar Typhimurium trimethylamine + oxidized electron donor + H2O
-
 ?
1.7.2.3 trimethylamine N-oxide + electron donor NADH as electron donor Salmonella enterica subsp. enterica serovar Typhimurium trimethylamine + oxidized electron donor + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.3 tetramer 4 * 84000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.3 45
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.2.3 75
-
 10 min, pH 7.3, quite stable at temperatures below Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 80
-
 10 min, pH 7.3, rapid loss of activity Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 90
-
 10 min, pH 7.3, complete loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.3 5.65
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.2.3 4.3
-
 not stable Salmonella enterica subsp. enterica serovar Typhimurium
1.7.2.3 6 8.5 stability peak at pH 7.3 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.3 NADH
-
 Salmonella enterica subsp. enterica serovar Typhimurium