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Literature summary extracted from

  • Bayer, M.; Walter, K.; Simon, H.
    Purification and partial characterization of a reversible artificial mediator accepting NADH oxidoreductase from Clostridium thermoaceticum (1996), Eur. J. Biochem., 239, 686-691.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 0.25
-
NAD+
-
Moorella thermoacetica
7.1.1.2 0.35
-
ubiquinone-0
-
Moorella thermoacetica
7.1.1.2 0.4
-
ferricyanide
-
Moorella thermoacetica
7.1.1.2 0.55
-
oxidized 2,6-dichlorophenolindophenol
-
Moorella thermoacetica
7.1.1.2 0.55
-
1,1'-carbamoylmethylviologen
-
Moorella thermoacetica
7.1.1.2 1
-
1,1'-carbamoylmethylviologen
-
Moorella thermoacetica
7.1.1.2 1.1
-
NADH
-
Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.2 Iron enzyme contains 10.6 atoms of iron and 12.7 atoms of sulfur per dodecamer Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.2 17000
-
12 * 17000, SDS-PAGE Moorella thermoacetica
7.1.1.2 200000
-
non-denaturing PAGE Moorella thermoacetica
7.1.1.2 210000
-
gel filtration Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Moorella thermoacetica
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
7.1.1.2 storage of 0.3 mg purified enzyme in 2 ml 0.1 M Tris/HCl, pH 8.0, for 24 h at 4°C, results in 15% inactivation under anaerobic conditions, 55% inactivation in presence of air Moorella thermoacetica

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2
-
Moorella thermoacetica

Storage Stability

EC Number Storage Stability Organism
7.1.1.2 -15°C, 0.07 mg/ml enzyme, 0.1 M Tris/HCl, pH 8.0, 72 h, under anaerobic conditions, 70% loss of activity Moorella thermoacetica
7.1.1.2 4°C, 0.07 mg/ml enzyme, 0.1 M Tris/HCl, pH 8.0, 72 h, under anaerobic conditions, 75% loss of activity Moorella thermoacetica
7.1.1.2 in liquid nitrogen, 5% loss of activity after 72 h Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + 1,1'-carbamoylmethylviologen
-
Moorella thermoacetica NAD+ + reduced 1,1'-carbamoylmethylviologen
-
r
7.1.1.2 NADH + anthraquinone-2,6-disulfonate
-
Moorella thermoacetica NAD+ + ?
-
?
7.1.1.2 NADH + FAD
-
Moorella thermoacetica NAD+ + FADH2
-
?
7.1.1.2 NADH + ferricyanide
-
Moorella thermoacetica NAD+ + H+ + ferrocyanide
-
?
7.1.1.2 NADH + oxidized 2,6-dichlorophenolindophenol
-
Moorella thermoacetica NAD+ + reduced 2,6-dichlorophenolindophenol
-
?
7.1.1.2 NADH + oxidized 2,6-dichlorophenolindophenol + H+
-
Moorella thermoacetica NAD+ + reduced 2,6-dichlorophenolindophenol
-
?
7.1.1.2 NADH + ubiquinone
-
Moorella thermoacetica NAD+ + ubiquinol
-
?
7.1.1.2 NADH + ubiquinone-0
-
Moorella thermoacetica NAD+ + ubiquinol-0
-
?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 dodecamer 12 * 17000, SDS-PAGE Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 AMAPOR
-
Moorella thermoacetica
7.1.1.2 artificial mediator accepting pyridine nuclöeotide oxidoreductase
-
Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.2 5
-
reduction of NAD+ with reduced methyl viologen Moorella thermoacetica
7.1.1.2 10
-
reaction with ferricyanide or 1,1'-carbamoylmethylviologen Moorella thermoacetica

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 NADH
-
Moorella thermoacetica