EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.1.1.2 | 0.25 | - |
NAD+ | - |
Moorella thermoacetica | |
7.1.1.2 | 0.35 | - |
ubiquinone-0 | - |
Moorella thermoacetica | |
7.1.1.2 | 0.4 | - |
ferricyanide | - |
Moorella thermoacetica | |
7.1.1.2 | 0.55 | - |
oxidized 2,6-dichlorophenolindophenol | - |
Moorella thermoacetica | |
7.1.1.2 | 0.55 | - |
1,1'-carbamoylmethylviologen | - |
Moorella thermoacetica | |
7.1.1.2 | 1 | - |
1,1'-carbamoylmethylviologen | - |
Moorella thermoacetica | |
7.1.1.2 | 1.1 | - |
NADH | - |
Moorella thermoacetica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.2 | Iron | enzyme contains 10.6 atoms of iron and 12.7 atoms of sulfur per dodecamer | Moorella thermoacetica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
7.1.1.2 | 17000 | - |
12 * 17000, SDS-PAGE | Moorella thermoacetica |
7.1.1.2 | 200000 | - |
non-denaturing PAGE | Moorella thermoacetica |
7.1.1.2 | 210000 | - |
gel filtration | Moorella thermoacetica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.1.1.2 | Moorella thermoacetica | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
7.1.1.2 | storage of 0.3 mg purified enzyme in 2 ml 0.1 M Tris/HCl, pH 8.0, for 24 h at 4°C, results in 15% inactivation under anaerobic conditions, 55% inactivation in presence of air | Moorella thermoacetica |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.1.1.2 | - |
Moorella thermoacetica |
EC Number | Storage Stability | Organism |
---|---|---|
7.1.1.2 | -15°C, 0.07 mg/ml enzyme, 0.1 M Tris/HCl, pH 8.0, 72 h, under anaerobic conditions, 70% loss of activity | Moorella thermoacetica |
7.1.1.2 | 4°C, 0.07 mg/ml enzyme, 0.1 M Tris/HCl, pH 8.0, 72 h, under anaerobic conditions, 75% loss of activity | Moorella thermoacetica |
7.1.1.2 | in liquid nitrogen, 5% loss of activity after 72 h | Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.1.2 | NADH + 1,1'-carbamoylmethylviologen | - |
Moorella thermoacetica | NAD+ + reduced 1,1'-carbamoylmethylviologen | - |
r | |
7.1.1.2 | NADH + anthraquinone-2,6-disulfonate | - |
Moorella thermoacetica | NAD+ + ? | - |
? | |
7.1.1.2 | NADH + FAD | - |
Moorella thermoacetica | NAD+ + FADH2 | - |
? | |
7.1.1.2 | NADH + ferricyanide | - |
Moorella thermoacetica | NAD+ + H+ + ferrocyanide | - |
? | |
7.1.1.2 | NADH + oxidized 2,6-dichlorophenolindophenol | - |
Moorella thermoacetica | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
7.1.1.2 | NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Moorella thermoacetica | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
7.1.1.2 | NADH + ubiquinone | - |
Moorella thermoacetica | NAD+ + ubiquinol | - |
? | |
7.1.1.2 | NADH + ubiquinone-0 | - |
Moorella thermoacetica | NAD+ + ubiquinol-0 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.1.1.2 | dodecamer | 12 * 17000, SDS-PAGE | Moorella thermoacetica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.1.1.2 | AMAPOR | - |
Moorella thermoacetica |
7.1.1.2 | artificial mediator accepting pyridine nuclöeotide oxidoreductase | - |
Moorella thermoacetica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.1.1.2 | 5 | - |
reduction of NAD+ with reduced methyl viologen | Moorella thermoacetica |
7.1.1.2 | 10 | - |
reaction with ferricyanide or 1,1'-carbamoylmethylviologen | Moorella thermoacetica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.2 | NADH | - |
Moorella thermoacetica |