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Literature summary extracted from

  • Fang, J.; Beattie, D.S.
    Novel FMN-containing rotenone-insensitive NADH dehydrogenase from Trypanosoma brucei mitochondria: isolation and characterization (2002), Biochemistry, 41, 3065-3072.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 diphenyl iodonium maximal inhibition after preincubation with NADH, more complete inhibition with the more hydrophobic electron acceptors such as ubiquinone-1 or ubiquinone-2 as electron acceptor compared to the more hydrophilic ones, such as ubiquinone-0 or dichloroindophenol Trypanosoma brucei
7.1.1.2 flavone insensitive to Trypanosoma brucei
7.1.1.2 rotenone rotenone-insensitive enzyme Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrial membrane inner membrane Trypanosoma brucei 31966
-
7.1.1.2 mitochondrion inner membrane Trypanosoma brucei 5739
-
7.1.1.2 mitochondrion loosly bound to inner mitochondrial membrane Trypanosoma brucei 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.2 33000
-
 2 * 33000, SDS-PAGE Trypanosoma brucei
7.1.1.2 65000
-
 gel filtration Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2
-
 Trypanosoma brucei

Reaction

EC Number Reaction Comment Organism Reaction ID
7.1.1.2 NADH + ubiquinone + 6 H+[side 1] = NAD+ + ubiquinol + 7 H+[side 2] ping-pong mechanism Trypanosoma brucei
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.2 additional information procyclic form of the organism Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 4.8
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + O2
-
 Trypanosoma brucei NAD+ + superoxide radical
-
 ?
7.1.1.2 NADH + oxidized 2,6-dichlorophenolindophenol + H+
-
 Trypanosoma brucei NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Trypanosoma brucei NAD+ + ubiquinol
-
 ?
7.1.1.2 NADH + ubiquinone-0
-
 Trypanosoma brucei NAD+ + ubiquinol-0
-
 ?
7.1.1.2 NADH + ubiquinone-1
-
 Trypanosoma brucei NAD+ + ubiquinol-1
-
 ?
7.1.1.2 NADH + ubiquinone-2
-
 Trypanosoma brucei NAD+ + reduced ubiquinol-2
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 dimer 2 * 33000, SDS-PAGE Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.2 6 6.5
-
 Trypanosoma brucei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
7.1.1.2 4.5 9.5 stable Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FMN enzyme contains noncovalently bound FMN Trypanosoma brucei
7.1.1.2 NADH
-
 Trypanosoma brucei