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Literature summary extracted from

  • Kashani-Poor, N.; Kerscher, S.; Zickermann, V.; Brandt, U.
    Efficient large scale purification of his-tagged proton translocating NADH:ubiquinone oxidoreductase (complex I) from the strictly aerobic yeast Yarrowia lipolytica (2001), Biochim. Biophys. Acta, 1504, 363-370.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrial membrane
-
 Yarrowia lipolytica 31966
-
7.1.1.2 mitochondrion membrane Yarrowia lipolytica 5739
-
7.1.1.2 mitochondrion constitutively high content Yarrowia lipolytica 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.2 NADH + ubiquinone Yarrowia lipolytica proton-translocating NADH-ubiquinone oxidoreductase is the largest multiprotein complex of the respiratory chain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Yarrowia lipolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 efficient large scale purification of His-tagged proton translocating complex Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 64
-
 NADH:hexamineruthenium(III)-chloride oxidoreductase activity Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + decylubiquinone n-decylubiquinone Yarrowia lipolytica NAD+ + decylubiquinol
-
 ?
7.1.1.2 NADH + hexamineruthenium(III)-chloride
-
 Yarrowia lipolytica NAD+ + ?
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Yarrowia lipolytica NAD+ + ubiquinol
-
 ?
7.1.1.2 NADH + ubiquinone proton-translocating NADH-ubiquinone oxidoreductase is the largest multiprotein complex of the respiratory chain Yarrowia lipolytica ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 NADH
-
 Yarrowia lipolytica